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Antibodies-Online/Cyclin A1 (CCNA1) (AA 1-465), (full length) (Active) protein (His tag)/ABIN2003721/100 μg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Antibodies-Online/Cyclin A1 (CCNA1) (AA 1-465), (full length) (Active) protein (His tag)/ABIN2003721/100 μg

ProteinName
CyclinA1(CCNA1)
  • fd14f08
  • wu:fd14f08
  • wu:fi20d12
  • zgc:111869
  • MGC81965
  • cyclin-A1
  • CCNA1
  • MGC132235
  • cyclin-A1-2
  • Cyclin-A1
  • ccna1
  • cyclin-A1-like
  • cyclinA1
  • cyclin-A1-2
  • LOC100147188
  • ccna1
  • CCNA1
  • CYCA1;2
  • Ccna1
  • LOC397885
ProteinCharacteristics
AA1-465,fulllength
2AA1-421
1AA1-391
1AA1-418
1AA1-464
1AA1-465
1AA183-430
1TranscriptVariant1
1fulllength
Origin
Human
5Human
1CrucianCarp(Carassiuscarassius)
1Mouse(Murine)
1Rat(Rattus)
1Xenopuslaevis
Source
BaculovirusinfectedInsectCells
3Yeast
2Escherichiacoli(E.coli)
2InsectCells
1HEK-293Cells
1Wheatgerm
ProteinType
Recombinant
BIOLOGicalActivity
Active
Purificationtag/Conjugate
ThisCyclinA1proteinislabelledwithHistag.
Application
FunctionalStudies(Func),SDS-PAGE(SDS)
Options
Bulkdiscount
Supplier
SupplierProductNo.
CharacteristicsTherecombinanthumanCCNA1consistsof483aminoacidsandpredictsamolecularmassof54.6kDa.Itmigratesasanapproximately50kDabandinSDS-PAGEunderreducingconditions.

ProteinStructure:ADNAsequenceencodingthefulllengthofhumanCCNA1isoforma(NP_003905.1)(Met1-Gln465)wasexpressed,withapolyhistidinetagattheN-terminus.
PredictedN-Term:His
Purity>96 %asdeterminedbySDS-PAGE
EndotoxinLevel<1.0EUperμgoftheproteinasdeterminedbytheLALmethod
ProductDetails:BiologicalActivityComment1.MeasuredbyitsbindingABIlityinafunctionalELISA.ImmobilizedhumanHis-CCNA1at10μg/ml(100μl/well)canbindbiotinylatedhumanCDK1.TheEC50ofbiotinylatedhumanCDK1is0.02-0.04μg/ml.
2.MeasuredbyitsbindingabilityinafunctionalELISA.ImmobilizedhumanHis-CCNA1at10μg/ml(100μl/well)canbindbiotinylatedhumanCDK2-His.TheEC50ofbiotinylatedhumanCDK2-Hisis0.07-0.15μg/ml.
BackgroundSynonyms:CT146
MolecularWeight54.6kDa,50kDa
NCBIAccessionNP_003905
PathwaysApoptosis,CellDivisionCycle,AMPKSignaling,MitoticG1-G1/SPhases,DNAReplication,MPhase,SynthesisofDNA
ApplicationNotesOptimalworkingdilutionshouldbedeterminedbytheinvestigator.
RestrictionsForResearchUseonly
FormatLyophilized
BufferLyophilizedfromsterile20 mMTris,500 mMNaCl, pH7.4
Normally5 %-8 %trehalose,mannitoland0.01 %Tween80areaddedasprotectantsbeforelyophilization.
HandlingAdviceAvoidrepeatedfreeze-thawcycles.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.
Storage-20°C,-80°C
StorageCommentStoreitundersterileconditionsat-20°Cto-80°C.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.Avoidrepeatedfreeze-thawcycles.

Shipping:Ingeneral,recombinantproteinsareprovidedaslyophilizedpowderwhichareshippedatambienttemperature.
Bulkpackagesofrecombinantproteinsareprovidedasfrozenliquid.Theyareshippedoutwithblueiceunlesscustomersrequireotherwise.
ExpiryDate12months
SupplierImages
image for Cyclin A1 (CCNA1) (AA 1-465), (full length) (Active) protein (His tag) (ABIN2003721)CyclinA1(CCNA1)(AA1-465),(fulllength)(Active)protein(Histag)
BackgroundpublicationsDiederichs,Bäumer,Ji,Metzelder,Idos,Cauvet,Wang,Möller,Pierschalski,Gromoll,Schrader,Koeffler,Berdel,Serve,Müller-Tidow:"IdentificationofinteractionpartnersandsubstratesofthecyclinA1-CDK2complex."in:TheJournalofbiologicalchemistry,Vol.279,Issue32,pp.33727-41,2004

Diederichs,Bäumer,Ji,Metzelder,Idos,Cauvet,Wang,Möller,Pierschalski,Gromoll,Schrader,Koeffler,Berdel,Serve,Müller-Tidow:"IdentificationofinteractionpartnersandsubstratesofthecyclinA1-CDK2complex."in:TheJournalofbiologicalchemistry,Vol.279,Issue32,pp.33727-41,2004(PubMed).

Yang,Müller,Huynh,Fung,Yee,Koeffler:"FunctionsofcyclinA1inthecellcycleanditsinteractionswithtranscriptionfactorE2F-1andtheRbfamilyofproteins."in:Molecularandcellularbiology,Vol.19,Issue3,pp.2400-7,1999

Yang,Müller,Huynh,Fung,Yee,Koeffler:"FunctionsofcyclinA1inthecellcycleanditsinteractionswithtranscriptionfactorE2F-1andtheRbfamilyofproteins."in:Molecularandcellularbiology,Vol.19,Issue3,pp.2400-7,1999(PubMed).

Yang,Morosetti,Koeffler:"CharacterizationofasecondhumancyclinAthatishighlyexpressedintestisandinseveralleukemiccelllines."in:Cancerresearch,Vol.57,Issue5,pp.913-20,1997

Yang,Morosetti,Koeffler:"CharacterizationofasecondhumancyclinAthatishighlyexpressedintestisandinseveralleukemiccelllines."in:Cancerresearch,Vol.57,Issue5,pp.913-20,1997(PubMed).

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