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Accelagen/TurboBiotinylation DTTM Kit/50X TurboBiotinylase DT (1 mg)
10X TurboBiotinylation Mix (1 ml)/B0112M

Accelagen TurboBiotinylation DT Kit, the best biotinylation kit
The Gene-to-Protein Company
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Protein PurificationBaculovirus ExpressionMammalian ExpressionE. coli ExpressionMolecular BiologyProducts

Products: Eznyme Toolbox: TurboBiotinylation DT Kit

TurboBiotinylation DTTM Kit

Catalog #:Size:Concentration: Price:
B0112P 50X TurboBiotinylase DT (0.1 mg) 10X TurboBiotinylation Mix (1 ml) 5 mg/ml $298
B0112M 50X TurboBiotinylase DT (1 mg) 10X TurboBiotinylation Mix (1 ml) 5 mg/ml $1,678
B0112L 50X TurboBiotinylase DT (5 mg) 10X TurboBiotinylation Mix (5 ml) 5 mg/ml $5,400
T0112B Bulk 5 mg/ml Inquiry
To Order: Please email PO to order@accelagen.com or fax PO to 888-239-6490
PRODUCT DETAILDATA SHEETPROTOCOL
Components and Formulation:
  1. 50X TurboBiotinylase DT at 5 mg/ml (80 µM) in Storage Buffer of 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM TCEP, 50% glycerol.
  2. 10X TurboBiotinylation Mix (50 mM Tris-HCl, pH 7.5, 100 mM MgCl2, 100 mM ATP, 5 mM biotin)
TurboBiotinylase Purity:
TurboBiotinylase DT is a stable ultra-pure recombinant E. coli BirA biotin ligase with dual GST- and His-tags. It is purified from E. coli and has a calculated molecular weight of 62.4 kDa. It is free of protease or nuclease contamination. It can be easily removed by affinity capture through its GST- or His-tag.
TurboBiotinylase Activity:
The efficiency of biotinylation can be measured by mass spectrometry. Biotinylation adds 226.31 Da to a protein.

Alternatively, biotinylation can be evaluated by streptavidin-agarose pull-down. A Coomassie stained gel will visually show the efficiency of biotinylation.

  1. Remove biotin and ATP from the biotinylation reaction sample by a spin desalting column.
  2. Save sample as Total (T)
  3. Incubate with streptavidin-agarose for 1 hour with agitation.
  4. Spin down the streptavidin-agarose.
  5. Save the supernatant as Unbound (U)
  6. Save the streptavidin-agarose as Bound (B)
  7. Run gel and stain with Coomassie Blue.

Protein Purification |Baculovirus Expression | Mammalian Expression |E. coli Expression |Molecular Biology | Proteins

tel: 858-678-8618fax: 888-239-6490 toll-free: 888-365-8085email: info@accelagen.comCopyright © 2020 Accelagen Inc.All rights reserved.

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