Description: HumanPAK1kinase(UniProt:Q13153;NCBI:NM_002576)belongstoafamilyofp21-activatedkinases.Theseserine/threoninekinasesserveastargetsforthesmallGTPbindingproteinsCdc42andRacandhavebeenimplicatedinawiderangeofBIOLOGicalactivities.PAK1kinaseregulatescellmotilityandmorphology.PAK1KDisarecombinantwildtypehumanPAK1kinasedomain(aminoacids248–545offull-lengthhumanPAK1)expressedinE.coliandpurifiedtonearhomogeneity.PAK1KDdoesnothavetheinhibitoryswitchdomainandhashighspecificactivity. MolecularMassandPhosphorylation: Thecalculatedmassis33,646.PAK1KDisfullyphosphorylatedatT423andpartiallyphosphorylatedatadditionalsiteswithmeasuredmassof33,728(1P),33,808(2P),33,889(3P),and33,968(4P). StABIlityandStorage: Stableat-70oCfor12monthsfromdateofreceipt.Proteinshouldbethawedonice.Proteincanbeflash-frozeninliquidnitrogenandstoredat-70oC. Applications: Enzymaticstudies,inhibitorscreen,andselectivityprofiling Highconcentrationavailableforcrystallization |
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