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Accelagen/PAK2KD (recombinant human PAK2 kinase domain)/K0105B/ Bulk

Description:
HumanPAK2kinase(UniProt:Q13177;NCBI:NM_002577)belongstoafamilyofp21-activatedkinases.Theseserine/threoninekinasesareregulatedbythesmallGTPbindingproteinsCdc42andRacandhavebeenimplicatedinawiderangeofBIOLOGicalactivities.PAK2isactivatedbyproteolyticcleavageduringcaspase-mediatedapoptosis,andmayplayaroleinregulatingtheapoptoticeventsinthedyingcell.

PAK2KDisarecombinantwildtypehumanPAK2kinasedomain(aminoacids227–524offull-lengthhumanPAK2)expressedinE.coliandpurifiedtonearhomogeneitywithhighspecificactivity.

MolecularMassandPhosphorylation:
Thecalculatedmassis33,510.PAK2KDisfullyphosphorylatedatT402andpartiallyphosphorylatedatadditionalsiteswithmeasuredmassof33,593(1P),33,673(2P),33,753(3P),and33,834(4P).

StABIlityandStorage:
Stableat-70oCfor12monthsfromdateofreceipt.Proteinshouldbethawedonice.Proteincanbeflash-frozeninliquidnitrogenandstoredat-70oC.

Applications:
Enzymaticstudies,inhibitorscreen,andselectivityprofiling
Highconcentrationavailableforcrystallization