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Acetylated-LDL-Saporin (acLDL-SAP) has been used effectively to eliminate microglia from primary cultures of rat hippocampal neurons, while having no deleterious effect on neurons or astroglia. After 24 hours exposure, 3 åµg/ml of acLDL-SAP selectively kills more than 90% of microglia cells. acLDL-SAP is excellent for removing contaminating macrophages from primary cultures to determine their role(s) in autoimmune diseases and in degenerative diseases such as Alzheimer’s.
Acetylated LDL-SAP eliminates scavenger receptor-positive cells. This includes the macrophage and its relative, the microglia. All other cells are left untouched. Not suitable for retrograde transport.
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