LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,r
ImmunogenPeptide(C)KNANFTGPNQTSSNS,correspondingtoaminoacidresidues21-35ofhumanα1B-adrenoceptor(Accession P35368).Extracellular,N-terminus.
Accession(Uniprot)NumberP35368
GeneID147
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse-14/15aminoacidresiduesidentical;rat-13/15aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
LabelATTO-488.Maximumabsorption501nm;maximumfluorescence523nm.Thefluorescenceisexcitedmostefficientlyinthe480–515nmrange.Thislabelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution50µldoubledistilledwater(DDW).
Antibodyconcentrationafterreconstitution1mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-018),andlivecellimaging(labeledantibody).
Applications:fc,ic,lci
Mayalsoworkin:ih
DirectflowcytometryDirectflowcytometryanalysisinratGH3pituitaryadenomacells:
___Cells.
___Cells+ Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488 Antibody(#AAR-018-AG),(10µg/5x105 cells).
Livecellimaging/ImmunocytochemistryExpressionofα1B-AdrenoceptorinratGH3cells
ImmunocytochemicalstainingofGH3cellswithAnti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody (#AAR-018-AG)(green).Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342(blue).
ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.
Minneman,K.P.(1988)Pharmacol.Rev.40,87.
Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.
ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
Adrenergicreceptorsaremembersofthe G-proteincoupledreceptor (GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.
Adrenoceptorsaredividedintothreetypes:α1,α2 andβadrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β3.1,2 Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.1,2
Allα1-adrenoceptors(α1-ARs)activatephospholipasesCandA2.3 Inadditiontomobilizingintracellularcalcium,theα1-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.4
α1B-Adrenoceptorpopulationsaregreatestinthespleen,kidney,cerebellum,andfetalbrain.5
α1B-Adrenoceptorcausescontractionofsmoothmusclecellsandtherebycontrolsvasculartone,bloodpressure,andacceleratesthedevelopmentofatherosclerosis.5
LyophilizedPowderControlAntigenIncluded
Lastupdate:24/03/2019
Anti-α1B–AdrenergicReceptor(extracellular)Antibody(#AAR-018)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα1B-adrenoceptor.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.Ithasbeendesignedtorecognizeα1B-adrenoceptorfromhuman,ratandmousesamples.
Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody(#AAR-018-AG)isdirectlylabeledwithanATTO-488fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABIlity.TheATTO-488labelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody hasbeentestedindirectflowcytometryapplicationsandisspeciallysuitedtoexperimentsrequiringsimultaneouslabelingofdifferentMarkers.
Forresearchpurposesonly,notforhumanuse
DirectflowcytometryDirectflowcytometryanalysisinratGH3pituitaryadenomacells:
___Cells.
___Cells+ Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488 Antibody(#AAR-018-AG),(10µg/5x105 cells).
Livecellimaging/ImmunocytochemistryExpressionofα1B-AdrenoceptorinratGH3cells
ImmunocytochemicalstainingofGH3cellswithAnti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody (#AAR-018-AG)(green).Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342(blue).
ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.
Minneman,K.P.(1988)Pharmacol.Rev.40,87.
Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.
