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Alomone/Anti-Adenosine A2A Receptor Antibody/AAR-002/0.2 ml

Cat#:AAR-002
  • LyophilizedPowder
  • AntigenIncl.
  • ShippedatRoomTemp.
  • Type:Polyclonal
    Source:Rabbit
    Reactivity:h,m,r
    Immunogen
    Peptide(C)RQLKQMESQPLPGER,correspondingtoaminoacidresidues201-215ofmouseadenosineA2A receptor(AccessionQ60613).3rdintracellularloop.
    Accession(Uniprot)NumberQ60613
    GeneID11540
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,human,andcanis–identical.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.75mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Controlantigenreconstitution100µldoubledistilledwater(DDW).
    Controlantigenstorageafterreconstitution-20°C.
    PreadsorptionControl1µgpeptideper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,ih,wb
    Mayalsoworkin:ifc,ip
    Westernblot
    Westernblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:
    1,2,5.Anti-Adenosine A2A Receptor Antibody(#AAR-002),(1:200).
    3,4,6.Anti-AdenosineA2A ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
    Immunohistochemistry
    ExpressionofAdenosine A2A Receptor in mousediagonalband
    ImmunohistochemicalstainingofmousediagonalbandusingAnti-Adenosine A2A ReceptorAntibody(#AAR-002).A2aR (green)appearsinthebroca ofindividualneurons(arrows)andinneuropil(asterisk). DAPIisusedasthecounterstain.
    ExpressionofAdenosine A2A Receptor inratlung
    ImmunohistochemicalstainingofparaffinemeddedratlungsectionsusingAnti-Adenosine A2A Receptor Antibody(#AAR-002),(1:50).A2aRisexpressedintherespiratoryepitheliumofthebronchioli(Br).Notethatsmoothmuscleandendotheliuminbloodvesselsarenegative.Hematoxilinisusedasthecounterstain.
    Immunocytochemistry
    Ratneurospheres(Benito-Munoz,M. etal. (2016) Glia 64, 1465.).
    References
    1. Okusa,M.D.(2002)Am.J.Physiol.282,F10.
    2. Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
    3. Nakata,H.(1989)J.Biol.Chem.264,16545.
    4. Cunha,R.A.(2001)NeuRochem.Int.38,107.
    5. Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
    6. Canals,M.etal.(2005)J.Neurochem.92,337.
    7. Ohta,A.andSitkovsky,M.(2001)Nature414,916.
    8. Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.
    Scientificbackground

    Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor(A1AR),AdenosineA2A Receptor(A2AAR),AdenosineA2B Receptor(A2BAR),andAdenosineA3 Receptor(A3AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH2 terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportantforbindingGproteins.1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs.1-3

    Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A2AARandtheinhibitoryA1AR.4 A2AARandA1ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA1ARiswidelydistributedthroughouttheCNS.5,6

    A2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.7 ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.7 ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues.8

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea25µlfreetrialsample!
    Lastupdate:17/02/2019

    Anti-Adenosine A2A ReceptorAntibody(#AAR-002)isahighlyspecificantibodydirectedagainstanepitopeofmouseprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,andimmunocytochemistryapplications.IthasbeendesignedtorecognizeA2aRfromhuman,rat,andmousesamples.

    Forresearchpurposesonly,notforhumanuse
    Westernblot
    Westernblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:
    1,2,5.Anti-Adenosine A2A Receptor Antibody(#AAR-002),(1:200).
    3,4,6.Anti-AdenosineA2A ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
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