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Alomone/Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody/AAR-003-F/2 x 50 µl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品

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Alomone/Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody/AAR-003-F/2 x 50 µl

Cat#:AAR-003-F

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h,m

Mayalsoworkin:r

Immunogen

PeptideKDSATNN(S)TEPWDGTTNESC,correspondingtoaminoacidresidues147-166ofhuman A_2BAR(Accession P29275).2^nd extracellularloop.

Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody -

Accession(Uniprot)NumberP29275

GeneID136

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyRat -14/19aminoacidresiduesidentical;mouse-13/19aminoacidresiduesidentical

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

LabelFluoresceinisothiocyanate(FITC).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-003),anddirectflowcytometry(labeledantibody).

Applications:fc

Mayalsoworkin:ic,lci

Directflowcytometry

DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

DirectflowcytometryofliveintactmouseJ774macrophagecells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

References

Peeters,M.C.etal.(2011)FASEBJ.25,632.
Sun,Y.andHuang,P.(2016)Front.Chem.4,37.

Scientificbackground

ExtracellularadenosineisaubiquitoussignalingmoleculethatmodulatesawidearrayofBIOLOGicalprocesses.Therearecurrentlyfourknownsub-typesofadenosinereceptors:A₁,A_2A,A_2B andA₃.

TheadenosineA_2B receptorisatypicalclassA,Gprotein-coupledreceptorwithseventransmembranesegments,anextracellularN-terminusandanintracellularC-terminus.AdenosineA_2BRcausesanincreaseinintracellularcAMPlevelsbycouplingmainlytoG_s proteins,resultingintheactivationofadenylylcyclase¹ andsubsequentactivationofPKAandothercAMPeffectorssuchasEpac.Inaddition,theA_2BRcouplestoG_q andactivatesthePLCpathwaythatmediatesseveralcrucialfunctionsofA_2BR.A_2BRalsocouplestotheMAPKandarachidonicacidsignalingpathwaysandregulatesmembraneionchannelsprobablythroughG-proteinβγsubunits.

MultiplemoleculesthatbindtoA_2BRhavebeenidentifiedinanefforttounderstandthereceptor’sfunctionandregulation.NFκB1/p105isamemberoftheNFκBfamilyofproteinsthatperformregulatoryfunctionsindiversebiologicalprocessessuchasinflammationandcellsurvivalanddifferentiation,aswellasinvariousdiseases.Interestingly,AlthoughA_2BRactivationbyadenosineproducespro-inflammatoryeffectsthereceptoralsobindstospecificsitesonp105,preventsthepoly-ubiquitinationanddegradationofthep105proteinandinhibitsNFκBactivationthusreducinginflammation.

A_2BRishighlyexpressedinvarioustypesoftumorcellsortissuesandpromotestumor-cellproliferation.A_2BRwasfoundtobeoverexpressedincolorectalcarcinomacellsandtissues,andinhibitionofA_2BRblockstheproliferationofcoloncancercells.A_2BRhasbeenimplicatedinthepathophysiologyofvasculardiseases,renaldiseaseanddiabetes².

LyophilizedPowderControlAntigenIncluded

Lastupdate:24/03/2019

Anti-AdenosineA_2B Receptor(extracellular)Antibody(#AAR-003)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimaging,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeA_2BARfromhuman,rat,andmousesamples.

Anti-Adenosine A_2B Receptor(extracellular)-FITCAntibody(#AAR-003-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.

Forresearchpurposesonly,notforhumanuse

Directflowcytometry

DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

DirectflowcytometryofliveintactmouseJ774macrophagecells:

^___Cellsalone.
^___Cells+RabbitIgGisotypecontrol-FITC.
^___Cells+Adenosine A_2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.

References

Peeters,M.C.etal.(2011)FASEBJ.25,632.
Sun,Y.andHuang,P.(2016)Front.Chem.4,37.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Alomone/Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody/AAR-003-F/2 x 50 µl

Applicationkey:

Speciesreactivitykey: