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Alomone/Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody/AAR-007-F/5 x 50 µl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品

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产品资料

Alomone/Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody/AAR-007-F/5 x 50 µl

Cat#:AAR-007-F

LyophilizedPowder

AntigenIncl.

ShippedatRoomTemp.

Type:Polyclonal

Source:Rabbit

Reactivity:h

Immunogen

PeptideCGQPKEGKNHSQG,correspondingtoaminoacidresidues146-158ofhumanadenosine A_2A receptor(AccessionP29274).Extracellular,2^ndloop.

Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody -

Accession(Uniprot)NumberP29274

GeneID135

PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.

HomologyHumanonly.

PurityAffinitypurifiedonimmobilizedantigen.

FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN₃.

SpecificityWillnotrecognizeratormousesamples.

LabelFluoresceinisothiocyanate(FITC).

StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.

Reconstitution50µldoubledistilledwater(DDW).

Antibodyconcentrationafterreconstitution1mg/ml.

StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).

ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.

Controlantigenreconstitution100µldoubledistilledwater(DDW).

Controlantigenstorageafterreconstitution-20°C.

PreadsorptionControl1µgpeptideper1µgantibody.

StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-007),anddirectflowcytometry(labeledantibody).

Applications:fc

Mayalsoworkin:ic,lci

Directflowcytometry

DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:

^___ Cells.
^___Cells+rabbitIgGisotypecontrol-FITC.
^___ Cells+Anti-HumanAdenosineA_2A Receptor(extracellular)-FITCAntibody (#AAR-007-F),(2.5µg).

DirectflowcytometryofliveintacthumanJurkatT-cellleukemiacells:

^___ Cells.
^___Cells+rabbitIgGisotypecontrol-FITC.
^___ Cells+Anti-HumanAdenosineA_2A Receptor(extracellular)-FITC Antibody (#AAR-007-F),(2.5µg).

References

Okusa,M.D.(2002)Am.J.Physiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545.
Cunha,R.A.(2001)NeuRochem.Int.38,107.
Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.
Canals,M.etal.(2005)J.Neurochem.92,337.
Ohta,A.andSitkovsky,M.(2001)Nature414,916.
Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.

Scientificbackground

Adenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart^1,2. Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors): AdenosineA₁ Receptor (A₁AR),AdenosineA_2A Receptor(A_2AAR), AdenosineA_2B Receptor (A_2BAR),and AdenosineA₃ Receptor (A₃AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH₂ terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportantforbindingGproteins.^1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs^1-3.

Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A_2AARandtheinhibitoryA₁AR.⁴ A_2AARandA₁ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A_2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA₁ARiswidelydistributedthroughouttheCNS^5,6.

A_2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.⁷ ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA_2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.⁷ ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA_2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues⁸.

LyophilizedPowderControlAntigenIncluded

Lastupdate:24/03/2019

Anti-HumanAdenosineA_2A Receptor(extracellular)Antibody (#AAR-007)isahighlyspecificantibodydirectedagainstanepitopeofhumanprotein.Theantibodycanbeusedinwesternblotandlivecellflowcytometryapplications.IthasbeendesignedtorecognizeA2aRfromhumansamplesonly.Theantibodywillnotrecognizethereceptorfrommouseorratsamples.

Anti-HumanAdenosineA_2A Receptor(extracellular)-FITC Antibody (#AAR-007-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.

Forresearchpurposesonly,notforhumanuse