LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,r
Mayalsoworkin:m
ImmunogenPeptide(C)KETGAFYGREFKTAK,correspondingtoaminoacidresidues216-230ofhumanA3AR(AccessionP0DMS8).3rdintracellularloop.
Accession(Uniprot)NumberP0DMS8
GeneID140
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,mouse -14/16aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.7mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,ih,wb
Mayalsoworkin:ifc,ip
WesternblotWesternblotanalysisofMalme-3Mcelllysate:
1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).
2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionofAdenosine A3 Receptorinratlung
Immunohistochemicalstainingofparaffin-embedded ratlungsectionsusingAnti-Adenosine A3 Receptor Antibody(#AAR-004),(1:50).Stainingisspecificforrespiratoryepithelialcellsofthebronchiole(arrows)andpneumonocytesinthealveolarwall(a).Hematoxilinisusedasthecounterstain.
ImmunocytochemistryExpressionofAdenosine A3 Receptorinahumanmelanomacellline
Immunocytochemicalstainingofparaformaldehyde-fixedandperme
ABIlized humanmelanomacells(A2058).A,D.Cellswerestainedusing
Anti-Adenosine A3 Receptor Antibody(#AAR-004),(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.B,E.Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342.C.MergedimageofpanelsAandB.F.MergedimageofpanelsDandE.
ReferencesOkusa,M.D.(2002)Am.J.Physiol.RenalPhysiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545
Baraldi,P.G.etal.(2006)Curr.Med.Chem.13,3467.
Headrick,J.P.etal.(2003)Am.J.Physiol.HeartCirc.Physiol.285,H1797.
M
ADI,L.
etal.(2004)
Clin.CancerRes.10,4472.
Ochaion,A.etal.(2007)Ann.Rheum.Dis.66,446.
ScientificbackgroundAdenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor (A1AR), AdenosineA2A Receptor (A2AAR),AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).Thevariousadenosinereceptorscanbedistinguishedonthebasisoftheirdistinctmolecularstructures,distincttissuedistributions,anddifferentialselectivityforadenosineanalogs.1-4 AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopimportantinbindingGproteins.1-3
ExpressionofA3ARhasbeenreportedinthebrain,kidney,liver,andheart.Itplaysaroleinmodulationofcerebralischemia,asthma,andcellgrowth.Inaddition,recentstudieshaveestablishedacardioprotectiveroleforA3AR.5 ExpressionofA3ARwasreportedtobeelevatedincanceroustissuesaswellasinauto-immuneinflammatorydiseases.6,7 ApatenthasbeenfiledfortheuseofA3ARandA3ARagoNISTasadiagnosticMarkerfortherapeutictreatmentsofcancerandotherdiseases.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:26/02/2019
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanintracellularepitopeofthehumanadenosineA3 receptor(A3AR).Anti-Adenosine A3 ReceptorAntibody(#AAR-004)canbeusedinwesternblotanalysis,aswellasimmunocytochemicalandimmunohistocemicalapplications,andhasbeendesignedtorecognizeA3ARfromhuman,rat,andmousesamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofMalme-3Mcelllysate:
1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).
2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
