LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:m,r
Mayalsoworkin:h
ImmunogenPeptide(C)DAGNSSWNGTEAPG,correspondingtoaminoacidresidues7-20ofratα2A-adrenoceptor(AccessionP22909).Extracellular,N-terminus.
Accession(Uniprot)NumberP22909
GeneID25083
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse-identical;human-13/14aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.85mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ih,wb
Mayalsoworkin:ic,ifc,ip,lci
WesternblotWesternblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:
1,2.Anti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).
3,4.Anti-α2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionofα2A-Adrenoreceptorinratcerebellum
ImmunohistochemicalstainingofratcerebellumfreefloatingfrozensectionusingAnti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020).A.α2A-Adrenoreceptorstaining(green)appearsinprocessesofBergmannglia(whitearrows)inthemolecularlayer(ML).B.Stainingofthesamesectionwithmouseantiglialfibrillaryacidicprotein(GFAP)(red),demonstratesexpressioninbothBergmanngliaandingranulelayer(GL)astrocytes.C.MergedimageofpanelAandBdemonstratingthatα2A-adrenoreceptorexpressionisrestrictedtoBergmannglia.DAPIisusedasthecounterstain(blue).
Expressionofα2A-Adrenoreceptorinrathypothalamus
ImmunohistochemicalstainingofratventromedialhypothalamusfreefloatingfrozensectionusingAnti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:250).A.α2A-Adrenoreceptorstaining(green)appearsinastrocyticfibers(arrows)originatingfromthewallofthe3rdventricle(3rdV).B.DAPIisusedasthecounterstain(blue).C.MergedimageofpanelsAandB.
ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
CivantosCalzada,B.andAleixandredeArtinano,A.(2001)Pharmacol.Res.44,195.
Bylund,D.B.(2005)Br.J.Pharmacol.144,159.
ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularN-terminus,andacytoplasmicC-terminus.
Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β31,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2.
Ingeneral,thepharmacologicalpropertiesofeachsubtypearequitehomogenousacrossdifferentspecies.However,thisisnotthecasefortheα2A subtypewhichwasfirstisolatedfromhumanplatelets3.Thissubtypeshowsdifferentpharmacologicalpropertiesfromthatofmouseandrat.Forthisreason,untilmoleculartechniquessignificantlyadvanced,itwasbelievedthathumanα2A andthatofmouseandrat(termedα2D)weretwodifferentsubtypes.Today,itisacceptedthatthesetwosubtypesareinfactonegeneproductandisgenerallytermedα2A.Oneofthemaindifferencesbetweenthissubtypefromthedifferentorganismsisitsaffinityforyohimbineandrauwolscine;ratandmouseα2A displayslessaffinitycomparedtohuman3,4.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:04/11/2018
Anti-α2A-AdrenergicReceptor(extracellular)Antibody(#AAR-020)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα2A-adrenoceptorfrommouse,rat,andhumansamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:
1,2.Anti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).
3,4.Anti-α2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
