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The LEGENDplex™ Rat Inflammation Panel is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 rat cytokines, including IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-17A, IL-18, IL-33, CXCL1 (KC), CCL2 (MCP-1), GM-CSF, IFN-γ and TNF-α. Most of cytokines in this panel are produced by innate immune cells, linking the innate and adaptive immunity and/or bystander cells. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum and plasma samples.
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