Description | Flavoproteinsubunitofsuccinatedehydrogenase(SDH)thatisinvolvedincomplexIIofthemitochondrialelectrontransportchainandisresponsIBLefortransferringelectronsfromsuccinatetoubiquinone(coenzymeQ)(Ref:SWISSPROT). |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(YRPVIDKTLNEADCAT)correspondingtoaregion(641-656aa)fromtheC-terminusofhumanSuccinatedehydrogenase[ubiquinone]flavoproteinsubunit(SDNA). |
Sequence | YRPVIDKTLNEADCAT |
AntibodyType | Antiserum |
OtherNames | Succinatedehydrogenase[ubiquinone]flavoproteinsubunit;mitochondrial;FlavoproteinsubunitofcomplexII;Fp;SDH2;SDHF;DHSA; |
Accession | P31040DHSA_HUMAN; |
Producedin | Rabbit |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0µg/mlisrecommendedforWB.HumanSDHA(precursor)hasapredictedlengthof664residuesandMWof73kDa.Aconcentrationof2.0µg/mlisrecommendedforIHCtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
AntibodyAgainst | Succinatedehydrogenase[ubiquinone]flavoproteinsubunit |
Cross-reactivity | Human(WB);rat(WB,IHC-P);predictedtoreactwithmouseduetosequencehomology; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenproteins.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
BlastURL | Clickhere |
Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
Appearance | Lyophilizedwhitepowder |
Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
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