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| Description | TNFAIP1isacomponentoftheBCR(BACURD2)E3ubiquitinligasecomplex(Ref:SWISSPROT). |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(NKYVQLNVGGSLYYTTVRALTRHDTM)correspondingtoaregion(27-52aa)fromtheN-terminusofhumantumornecrosisfactor,alpha-inducedprotein1,endothelial(TNFAIP1). |
| Sequence | NKYVQLNVGGSLYYTTVRALTRHDTM |
| AntibodyType | Antiserum |
| OtherNames | BTB/POZdomain-containingadapterforCUL3-mediatedRhoAdegradationprotein2;hBACURD2;BTB/POZdomain-containingproteinTNFAIP1;ProteinB12;TNFAIP1;BACURD2;EDP1; |
| Accession | Q13829BACD2_HUMAN; |
| Producedin | Rabbit |
| Applications | Immunohistochemistry(IHC),Immunocytochemistry(ICC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanTNFAIP1hasapredictedlengthof316residuesandMWof36kDa.Aconcentrationof0.25-0.5µg/mlisrecommendedforIHCtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Aconcentrationof0.25-0.5µg/mlisrecommendedforIHCtodetecttheproteininfrozentissues.Aconcentrationof0.25-0.5µg/mlisrecommendedforICC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| AntibodyAgainst | Tumornecrosisfactor,alpha-inducedprotein1,endothelial |
| Cross-reactivity | Human;rat;predictedtoreactwithmouseduetosequencehomology; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenproteins.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| BlastURL | Clickhere |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Appearance | Lyophilizedwhitepowder |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |
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