Description | TheproteinidentifiedasCD44isalsoknownasthereceptorforhyaluronicacid(HA).Theproteinmediatescell-cellandcell-matrixinteractionsthroughitsaffinityforHA,andpossIBLyalsothroughitsaffinityforotherligandssuchasosteopontin,collagens,andmatrixmetalloproteinases(MMPs).AdhesionwithHAplaysanimportantroleincellmigration,tumorgrowthandprogression.Incancercells,CD44mayplayanimportantroleininvadopodiaformation,anditalsoisinvolvedinlymphocyteactivation,recirculationandhoming,andinhematopoiesis.AlteredexpressionordysfunctionofCD44causesnumerouspathogenicphenotypes.CD44hasgreatproteinheterogeneityduetonumerousalternativesplicingandpost-translationalmodificationevents.Biosensis"anti-CD44rabbitpolyclonalantibodyisanewantibodymadeagainstasyntheticpeptideepitopeneartheveryC-terminus,cytoplasmicdomainofhumanCD44.Theantibodyhasbeenshowntoreactwithmultiplespeciesincludinghuman,mouseandrat,inbothwesternblotandimmunohistochemistryapplicationsincludingformalinfixed,paraffinembeddedtissues.Seeapplicationsdetailforspecificuseinstructions. |
BatchNo. | seeviallabel |
Unitsize | 100µg |
Antigen | AsyntheticpeptidecorrespondingtoasequenceattheC-terminusofhumanCD44cytoplasmicdomainaa728-742.Theimmunogendiffersbyonlysingleaminoacidbetweenhuman,mouseandratproteins. |
Sequence | DETRNLQNVDMKIGV |
AntigenLocation | cytoplasmic,C-terminus |
AntigenLength | 15aa |
AntibodyType | polyclonal |
Isotype | IgG |
OtherNames | CDw44,Epican,ExtracellularmatrixreceptorIII,ECMR-III,GP90lymphocytehoming/adhesionreceptor,HUTCH-I,Heparansulfateproteoglycan,Hermesantigen,Hyaluronatereceptor,Phagocyticglycoprotein1,PGP-1,PhagocyticglycoproteinI,PGP-I,CD_antigen:CD44 |
Accession | P16070 |
Producedin | Rabbit |
MolecularWeight | Variabledependinguponsplicing,80-to250-kDa |
Purity | Affinitypurifiedoverpeptide |
Applications | WB:Westernblot:reducedRIPAsamplesormembranepreparations(0.1-.5ug/mL)dependentuponantigenloadandantibodyincubationtimesanddetectionsubstrateused. IH:frozenoracetonefixedtissue:0.5-1.0ug.mL,permeABIlizationisnecessaryasepitopeisinternal IH(P):1-2ug/mLwithHEIRtreatmentrequired. |
Form | lyophilized. |
Appearance | Eachvialcontains100ugpurifiedantibody,and5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3. |
Reconstitution | 0.2mlofdistilledwaterwillyieldaconcentrationof500μg/ml. |
Storage | At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobedividedintoaliquotsandstoredfrozenat-80˚Cforalongertime.Avoidrepeatedfreezingandthawing. |
ExpiryDate | 12monthsafterpurchase. |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn