Description | Calcium/calmodulin-stimulatedproteinkinaseII(CaMKII)iscomposedoffourdifferentchains(alpha,beta,gamma,anddelta)andisabundantlyexpressedinneurons.CaMKIIisinvolvedinregulatingmanyaspectsofneuronalfunction,includingneurotransmittersynthesisandrelease,modulationofionchannelactivityandcellulartransport.TheenzymaticfunctionofCaMKIIisregulatedbyitsmultiplephosphorylationsitesandtargetingtosub-cellularlocationsthroughinteractionswithproteinbindingpartners.PhosphorylationofThr253hasbeenidentifiedinvivoandfoundtoaltertheinteractionofCaMKIIwithbindingpartners,butnotchangeitsenzymaticactivity.Thus,phosphorylationofThr253issuggestedtomodulatefunctionalresponsesbasedonitsbindingpartnerandsubsequentlyitssub-cellularlocalization. |
BatchNo. | Seeproductlabel |
Unitsize | 100uL |
Antigen | Asyntheticpeptide(NKMLpTINPSC)correspondingtothesequencearoundThr253(AA249-257)inalpha-CaMKIIwassynthesized,purifiedto95%puritybyHPLC,analyzedbymassspectroscopyandcoupledtodiphtheriatoxoid. |
AntibodyType | Polyclonal |
Accession | P11275KCC2A_RAT |
Producedin | Rabbit |
Purity | Wholeserum |
Applications | WesternBlotting(1:2001:1000).Otherapplicationshavenotbeentested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Rat |
Cross-reactivity | Nottested.Predictedfromgeneanalysistoreactwithhumanandmousealpha-CaMKII. |
Form | Lyophilized |
Reconstitution | Reconstitutein100uLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.SkeldingKAetal(2012)JJCerebBloodFlowMetab32(12),2181-2192. 2.SkeldingKAetal(2010)CellSignal22(5),759-769. 3.GurdJWetal(2008)BrainRes1218,158-165. 4.MiguesPVetal(2006)JNeuRochem98(1),289-299. |
GeneralReferences | 1.SkeldingKAet(2011)CellCycle10(4),631-639. 2.DosemeciA&JaffeH(2010)BiochemBiophysResCommun391(1),78-84. 3.SkeldingKA&RostasJAP(2009)NeurochemRes34,1792-1804. 4.Dosemeci,A.etal(1994)JBiolChem269(50),31330-31333. |
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