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Biosensis/Rabbit antibody to phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha (alpha-CaMKII, Thr253)188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Biosensis/Rabbit antibody to phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha (alpha-CaMKII, Thr253)

DescriptionCalcium/calmodulin-stimulatedproteinkinaseII(CaMKII)iscomposedoffourdifferentchains(alpha,beta,gamma,anddelta)andisabundantlyexpressedinneurons.CaMKIIisinvolvedinregulatingmanyaspectsofneuronalfunction,includingneurotransmittersynthesisandrelease,modulationofionchannelactivityandcellulartransport.TheenzymaticfunctionofCaMKIIisregulatedbyitsmultiplephosphorylationsitesandtargetingtosub-cellularlocationsthroughinteractionswithproteinbindingpartners.PhosphorylationofThr253hasbeenidentifiedinvivoandfoundtoaltertheinteractionofCaMKIIwithbindingpartners,butnotchangeitsenzymaticactivity.Thus,phosphorylationofThr253issuggestedtomodulatefunctionalresponsesbasedonitsbindingpartnerandsubsequentlyitssub-cellularlocalization.
BatchNo.Seeproductlabel
Unitsize100uL
AntigenAsyntheticpeptide(NKMLpTINPSC)correspondingtothesequencearoundThr253(AA249-257)inalpha-CaMKIIwassynthesized,purifiedto95%puritybyHPLC,analyzedbymassspectroscopyandcoupledtodiphtheriatoxoid.
AntibodyTypePolyclonal
AccessionP11275KCC2A_RAT
ProducedinRabbit
PurityWholeserum
ApplicationsWesternBlotting(1:200–1:1000).Otherapplicationshavenotbeentested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityRat
Cross-reactivityNottested.Predictedfromgeneanalysistoreactwithhumanandmousealpha-CaMKII.
FormLyophilized
ReconstitutionReconstitutein100uLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
SpecificReferences1.SkeldingKAetal(2012)JJCerebBloodFlowMetab32(12),2181-2192.
2.SkeldingKAetal(2010)CellSignal22(5),759-769.
3.GurdJWetal(2008)BrainRes1218,158-165.
4.MiguesPVetal(2006)JNeuRochem98(1),289-299.
GeneralReferences1.SkeldingKAet(2011)CellCycle10(4),631-639.
2.DosemeciA&JaffeH(2010)BiochemBiophysResCommun391(1),78-84.
3.SkeldingKA&RostasJAP(2009)NeurochemRes34,1792-1804.
4.Dosemeci,A.etal(1994)JBiolChem269(50),31330-31333.
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