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| Description | Glialfibrillaryacidicprotein(GFAP)isapprox.50kDaintra-cytoplasmicfilamentousproteinoftheCytoskeletoninastrocytes.Duringthedevelopmentofthecentralnervoussystem,itisacell-specificMarkerthatdistinguishesastrocytesfromotherglialcells.GFAPimmunoreactivityhasbeenshowninimmatureoligodendrocytes,epiglotticcartilage,pituicytes,papillarymeningiomas,myoepithelialcellsofthebreastandinnon-CNS:Schwanncells,salivaryglandneoplasms,entericgliacells,andmetastasizingrenalcarcinomas. |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µl |
| Antigen | RecombinantGFAP(expressedinE.coli)andnativebovineGFAP |
| Isotype | IgY |
| OtherNames | Astrocyte;Glialfibrillaryacidicprotein;GFAP |
| Accession | P14136GFAP_HUMAN;Q28115GFAP_BOVIN; |
| Producedin | Chicken |
| MolecularWeight | Onwesternblotsofbrainorspinalcordhomogenateexpecttoseeabandat55kDaandanotheratabout48kDa,apparentlyabreakdownproductoftheprimaryband. |
| Applications | WesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:50,000isrecommendedforWB.HumanGFAPhasapredictedlengthof432residuesandaMWof50kDa.Adilutionof1:1000usingfluorescentsecondaryantibodiesor1:5000usingperoxidaseorotherenzyme-linkedmethodsisrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. ICC:1:1000-1:20,000+,4%PFAfixedcellsinculture,3hr-o/nincubations,optimizationisamustinmostcasesbecauseoftheantibodyhighreactivitytoGFAP IHC:4%Frozentissues,permeABIlized;IH(P):capable,HEIRtreatmenttypicallynecessary;ChickenIgYcanbindnon-specificallytotissues,specialchickenblocksolutionsavailableelsewherearerecommendedifusedontissues.Dilutions1:5000orgreater;optimizationontime,methodrequiredforbestresults. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB. |
| AntibodyAgainst | GlialFibrillaryAcidicProtein |
| Cross-reactivity | Human,Rat,Mouse,Feline.Predictedtoreactwithothermammals. |
| BlastURL | Clickhere |
| Form | Lyophilisedwith5%trehalose.TheIgYpreparationisataconcentrationof~25mg/mLtotalprotein. |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| GeneralReferences | 1.BrennerM.etal(2001)MutationsinGFAP,encodingglialfibrillaryacidicprotein,areassociatedwithAlexanderdisease.NatGenet.2001Jan;27(1):117-20. |
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