Description | Myelinisamembranecharacteristicofthenervoustissueandfunctionsasaninsulatortoincreasethevelocityofthestimulibeingtransmittedbetweenanervecellbodyanditstarget.Myelinisolatedfromhumanandbovinenervoustissueiscomposedofapproximately80%lipidand20%protein,and30%oftheproteinfractionconstitutesmyelinbasicprotein(MBP).MBPisan"intrinsicallyunstructured"proteinwithahighproportion(approximately75%)ofrandomcoil,butpostulatedtohavecoreelementsofbeta-sheetandalpha-helix.MBPisamajorproteininCNSmyelinandisexpressedspecificallyinthenervoussystem.AdetailedimmunochemicalexaminationofmonoclonalandpolyclonalantibodyresponsestoMBPanditspeptideshasrevealedtheexistenceofasmanyas27antigenicdeterminants,manyofthemconformational.TopologicalmappingofthepotentialantigenicdeterminantsontoamodelofMBPsecondarystructureplacesthesedeterminantswithin11separateregionsofthemolecule,includingthoseportionsthathavebeenfoundtobeencephalitogenic.Themessageformyelinbasicproteinisselectivelytranslocatedtotheendsofthecellprocesses.Immunizationwithmyelin-associatedantigensincludingMBPsignificantlypromotesrecoveryafterspinalcordcontusioninjuryintheratmodel.FUNCTION:Is,withPLP,themostabundantproteincomponentofthemyelinmembraneintheCNS.HasaroleinboththeformationandstABIlizationofthiscompactmultilayerarrangementofbilayers.Eachsplicevariantandchargeisomermayhaveaspecializedfunctionintheassemblyofanoptimized,biochemicallyfunctionalmyelinmembrane(Bysimilarity).SUBUNIT:Homodimer(Bysimilarity).SUBCELLULARLOCATION:Myelinmembrane;peripheralmembraneprotein;cytoplasmicside.Cytoplasmicsideofmyelin.TISSUESPECIFICITY:Foundinboththecentralandtheperipheralnervoussystem. |
BatchNo. | Seeproductlabel |
Unitsize | 50µl |
Antigen | ThreepeptidesequencesconservedinhigherverterbrateMBPprotein. |
Isotype | IgY |
OtherNames | MyelinBasicProtein;MyelinA1protein;Myelinmembraneencephalitogenicprotein; |
Accession | P02686MBP_HUMAN; |
Producedin | Chicken |
Applications | WesternBlotting(WB),Immunocytochemistry(IC)andImmunohistochemistry(IH).TherecommendeddilutionforWBis1:5,000-10,0000and1:500-1,000forICandIH.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB.Thisantibodystainsaprominentbandatapprox.20kDa.Asuitablecontroltissueisratspinalcordorperipheralnervehomogenate.ThemajorisoformsofMBPrunasacloselyspaceddoubleof22kDaand18kDa. |
AntibodyAgainst | MyelinBasicProtein |
Cross-reactivity | Hu,Rat,Ms,Bov.Predictedtoreactwithothermammaliantissuesduetosequencehomology. |
BlastURL | Clickhere |
Form | Lyophilisedwith5%trehalose |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.RangarajuS.etal(2009)Moleculararchitectureofmyelinatedperipheralnervesissupportedbycalorierestrictionwithaging.AgingCell.2009Apr;8(2):178-91. |
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