Description | Neurofilamentsarecomposedofthreeintermediatefilamentproteins:light(~68kDa),medium(~160kDa)andheavy(~200kDa),whichareinvolvedinthemaintenanceoftheneuronalcaliber.Neurofilamentlight(NF68orNF-L)isthemostabundantofthethreeproteins. |
BatchNo. | Seeproductlabel |
Unitsize | 50µl |
Antigen | PurifiedporcineNF-LfromspinalcordandrecombinantNF-L. |
Isotype | IgY |
OtherNames | NF-L;NF68;NEFL;Neurofilamentlightpolypeptide;NFL; |
Accession | P02547NFL_PIG;P07196NFL_HUMAN; |
Producedin | Chicken |
Applications | WesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:1,000-1:5,000isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyIC. |
AntibodyAgainst | NeurofilamentLight |
Cross-reactivity | Hu,Rat,Ms,Fel,Chk.Predictedtoreactwithothermammaliantissuesduetosequencehomology. |
BlastURL | Clickhere |
Form | Lyophilisedwith5%trehalose |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.RangarajuS.etal(2009)Moleculararchitectureofmyelinatedperipheralnervesissupportedbycalorierestrictionwithaging.AgingCell.2009Apr;8(2):178-91. |
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