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| Description | Neurofilamentsarecomposedofthreeintermediatefilamentproteins:light(~68kDa),medium(~160kDa)andheavy(~200kDa),whichareinvolvedinthemaintenanceoftheneuronalcaliber.NeurofilamentmediumrunsonSDS-PAGEgelsintherange145-170kDa,withsomevariationindifferentspecies. |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µl |
| Antigen | RecombinantfusionproteincontainingtheextremeC-terminalsegmentofratNF-M. |
| Isotype | IgY |
| OtherNames | Neurofilamentmediumpolypeptide;NF-M;160kDaneurofilamentprotein;Neurofilament3;NeurofilamenttripletMprotein;Nefm;Nef3;Nfm; |
| Accession | P12839NFM_RAT; |
| Producedin | Chicken |
| Applications | WesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:1,000-1:2,000isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | SpecificallyrecognizesthemediumneurofilamentsubunitNF-LinWB.Bandappearsat~145kDainWBfromrodentand~160kDaforhumanandbovineWB. |
| AntibodyAgainst | NeurofilamentMedium |
| Cross-reactivity | Hu,Rat,Ms,Fel,Chk.Predictedtoreactwithothermammaliantissuesduetosequencehomology. |
| BlastURL | Clickhere |
| Form | Lyophilisedwith5%trehalose |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | 1.JarjourA.A.etal(2007)Maintenanceofaxo-oligodendroglialparanodaljunctionsrequiresDCCandnetrin-1.JNeurosci.2008Oct22;28(43):11003-14. 2.RangarajuS.etal(2009)Moleculararchitectureofmyelinatedperipheralnervesissupportedbycalorierestrictionwithaging.AgingCell.2009Apr;8(2):178-91. 3.PearseD.D.etal(2007)TransplantationofSchwanncellsand/orolfactoryensheathinggliaintothecontusedspinalcord:Survival,migration,axonassociation,andfunctionalrecovery.Glia.2007Jul;55(9):976-1000. 4.ShawG.etal(2004)CharacterizationofthebovineneurofilamentNF-MproteinandCDNAsequence,andidentificationofinvitroandinvivocalpaincleavagesites.BiochemBiophysResCommun.2004Dec10;325(2):619-25. |
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