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| Description | Calbindin-Disahighlyconserved28kDaproteinthatbelongstoafamilyoflowmolecularweightcalcium-bindingproteins(CaBPs).Calbindin-Dhas4activecalciumbindingdomains.Itplaysaroleintheregulationofcalciumandcalciumrelatedsignallinginneuronsandduringembryodevelopment.Thisantibodybindsonlytocalbindin-D-28Kandnotwithotherfamilymemberssuchascalretininandcalbindin-D-9K. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | BovinekidneyCalbindin-D |
| Clone | CB-D7 |
| OtherNames | CALB1_BOVIN;CALB1; |
| Accession | P04467CALB1_BOVIN |
| Producedin | Mouse |
| Purity | IgG1 |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.BovineCalbindin-Dhasapredictedlengthof261residuesandaMWof28kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectCalbindin-Dinformalinfixedandparaffinembeddedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| SpeciesAgainst | Theantibodyworksonratandisexpectedtoworkonhuman,mouseandbovine. |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase. |
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