企业QQ
官方微信
| Description | CloneOX-42recognisestheratequivalentofhumanCD11bandsharesacommonepitopewithCB11c(integrinaphaMandalphaXchains).(PMID:1672643;TamataniTetal1991).CD11bis asingle-passtypeImembraneproteinthatbelongstotheintegrinalphachainfamily.CD11bispredominantlyexpressedinmonocytesandgranulocytesandisimplicatedinvariousadhesiveinteractionsofmonocytes,macrophagesandgranulocytesaswellasinmediatingtheuptakeofcomplement-coatedparticles(Ref:SWISSPROT).CD11bisalsofrequentlyusedasamicroglialMarkerallowingtodistinguishbetweenquiescentandactivatedmicrogliabasedontheintensityofCD11bstaining.MoreovertheOX-42monoclonalantibodyspecificallybindstotheCR3complement(C3bi)receptorfoundonmostmonocytes,granulocytes,macrophages,dendriticcells,andmicroglia.OX-42antibodyinhibitsC3bibindingactivity. CD11b,alsoknownasintegrinalphaMorMac-1,andisacomponentofcomplementreceptor3(CR3).CD11c,alsoknownasintegrinalphaX,andisacomponentofcomplementreceptor4(CR4).Integrinalpha-X/beta-2isareceptorforfibrinogen.CD11bandCD11careexpressedonimmunecellssuchasmacrophages,monocytes,granulocytes,anddendriticcells.OX42hasalsobeenshowntodetectmicrogliainthebrain,aswellascellsoftheliverandepidermis. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Ratperitonealmacrophages,wholecells.(Robinson,APetalImmunology198657239-247) |
| AntibodyType | Mousemonoclonal |
| Isotype | IgG2a,kappa |
| Clone | OX42,OX-42 |
| OtherNames | CD11b;CD11B;CD11antigen-likefamilymemberB;ITGAM;Integrinbeta2alphasubunit; CD11c; |
| Accession | Q9JI30Q9JI30_RAT |
| Producedin | Mouse |
| MolecularWeight | Underreducingconditionsofnativeimmunoprecipitatedproteinstwomajorpeptidesof163kDaand100kDaandaminor135kDapeptideareseen. |
| Purity | ProteinGpurifiedhybridomasupernatants. |
| Biol.activity | OX-42antibodyinhibitsC3bibindingactivity(BDraskovic-PavlovicetalImmunology199996:83-89). |
| Applications | FC:FlowCytometry:Unfixedcellspreferred,acetonefixedorquicklyfixed1%PLPfixedcellscanbeused. IH:Immunohistochemicalstudiesofratfreshfrozentissuesectionsandparaffin-embeddedtissuesectionsfollowingeitherperiodate-lysine-paraformaldehyde(PLP)fixation,oracetone.WorksonverylightlyPFAfixed,frozentissues.(perfusiononly4%PFA10-15"nopost-fix).Epitopecanbesensitivetofixation.Dilutionsdetectionmethoddependent1:100to1:200recommended. IC:Unfixedpreferred,oracetonefixedcells;5-10",2%PLPfixedcells,1-2ug/ml.Dilutionisdetectionmethoddependent. Immunoprecipitation:userabbitanti-mouseoranti-mouseIgGbeadsforcaptureonly.TheuseofproteinAorproteinGisnotrecommended.1-5ug/mLinrestrictedvolumes. ClonedoesnotworkintrADItionalreducedwesterns.Useimmunoprecipitationtoresolvereactiveproteinbands. Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | CloneOX-42recognisestheratequivalentofhumanCD11bandsharesacommonepitopewithCB11c(integrinaphaMandalphaXchains).(PMID:1672643;TamataniTetal1991). Immunoprecipitatesthreepolypeptidesof160kDa,103kDaand95kDaandafainterbandmayalsobeseenat133kDaundernon-reducingconditions.Iftheimmunoprecipitatedproteinsarereduced,twomajorpeptidesof163kDaand100kDaandaminor135kDapeptideareseen. |
| SpeciesAgainst | Rat(A.P.Robinson,etalImmunology198657239-247,originalpaper) |
| Cross-reactivity | Mis-informationexistsconcerningreactivitytomouseandhumanCD11b/cwithOX-42fromvariousvendors.BiosensishasnotverifiedthatOX42reactswithmouseandhuman,andONLYrecommendsthecloneonlyforratastheoriginalpaperandmostpapersusetheOXfamilyofclonesonrat. |
| Form | LyophilizedfromPBScontainingnopreservatives. |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial.Finalsolutionwillcontainnopreservatives.Observesteriletechniqueandproperhandlingforbestresults. |
| Storage | 12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | 1.RanaI.etal(2010)MicrogliaactivationinthehypothalamicPVNfollowingmyocardialinfarctionBrainRes.Apr22;1326:96-104. |
| References | 1.ZilkaN.etal(2009)HumanmisfoldedtruncatedtauproteinpromotesactivationofmicrogliaandleukocyteinfiltrationinthetransgenicratmodeloftauopathyJNeuroimmunol.Apr30;209(1-2):16-25.(activatedmicroglia) 2.BlackbeardJ.etal(2007)Quantificationoftheratspinalmicroglialresponsetoperipheralnerveinjuryasrevealedbyimmunohistochemicalimageanalysisandflowcytometry.JNeurosciMethods.Aug30;164(2):207-17. 3.EljaschewitschE.etal(2006)TheendocannabinoidanandamideprotectsneuronsduringCNSinflammationbyinductionofMKP-1inmicroglialcellsNeuron.Jan5;49(1):67-79(activatedmicroglia) 4.RobinsonA.P.,WhiteT.M.andMasonD.W.(1986)MacrophageheterogeneityintheratasdelineatedbytwomonoclonalantibodiesMRCOX-41andMRCOX-42,thelatterrecognizingcomplementreceptortype3Immunology.1986Feb;57(2):239-47.(originalpaper) |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn