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| Description | CD4isasingle-passtypeImembraneproteinexpressedonthesurfaceofThelper/inducercells,regulatoryTcells,monocytes,macrophages,dendriticcellsandmicroglia. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | CD4transfectedmouseT-cellhybridoma3DTfollowedbyCD4+humanT-cellCEMcellline |
| Clone | CA-4 |
| OtherNames | T-cellsurfaceglycoproteinCD4;T-cellsurfaceantigenT4/Leu-3;W3/25antigen;CD4mut; |
| Accession | P01730CD4_HUMAN;P05540CD4_RAT; |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC-Frozen).Aconcentrationof1.0µg/mlisrecommendedtodetectCD4informalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Thespecificityofthisantibodyhasbeenconfirmedbyimmunohistochemistryagainsttheantigen. |
| Cross-reactivity | Human;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpracticeisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Liquid(0.5ml).50%glycerol,0.9mgNaCland0.2mgNa2HPO4 |
| Reconstitution | TheliquidformulationshouldbedilutedinPBS(pH7.4) |
| Storage | Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.AlmoldaB.etal.CD4microglialexpressioncorrelateswithspontaneousclinicalimprovementintheacuteLewisratEAEmodel.JNeuroimmunol.2009Apr30;209(1-2):65-80. 2.JordanC.A.etal.Infectionofbrainmicroglialcellsbyhumanimmunodeficiencyvirustype1isCD4dependent.JVirol.1991Feb;65(2):736-42. |
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