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| Description | CD8isacellsurfaceglycoproteinfoundonmostcytotoxicTlymphocytes.Theantigeniseitherahomodimercomposedoftwoalphachainsoradisulphide-linkedheterodimercomposedofonealphaandonebetachain. Atleast2isoformsoftheCD8alphachainand6isoformsofthebetachainareproducedbyalternativesplicing. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | HumanthymocytesfollowedbyperipheralbloodT-cells |
| Clone | CA-8 |
| OtherNames | T-cellsurfaceglycoproteinCD8alphachain;T-lymphocytedifferentiationantigenT8/Leu-2;CD8a;MAL;T-cellsurfaceglycoproteinCD8betachain;CD8b;CD8B;CD8B1; |
| Accession | P01732CD8A_HUMAN;P10966CD8B_HUMAN; |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC-Frozen).Aconcentrationof1.0µg/mlisrecommendedtodetectCD8informalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Thespecificityofthisantibodyhasbeenconfirmedbyimmunohistochemistryagainsttheantigen. |
| Cross-reactivity | Human;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
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