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| Description | Fibrillarinispartofthesmallsubunitprocessomecomplex,involvedintheprocessingofpre-18SribosomalRNA.Nop1pistheyeasthomologueoffibrillarin.Fibrillarin/Nop1pisextraordinarilyconserved,sothattheyeastandhumanproteinsare67%identical,andthehumanproteincanfunctionallyreplacetheyeastprotein.Thismeansthatsuitablycross-reactiveantibodiestoNop1p/fibrillarinsuchasthisantibodycanbeusedtorevealnucleoliandstudyfibrillarin/Nop1pinalleukaryotesandarcheatestedtodate.ThisantibodyisbecomingwidelyusedasaconvenientMarkerfornucleoliinawidevarietyofspecies. |
| BatchNo. | Seeproductlabel. |
| Unitsize | 250µL |
| Antigen | Yeastnuclearpreparations.Hybridomaswerescreenedbyimmunofluorescenceonyeastcellsandbywesternblottingonyeastproteinhomogenates(S.cerevisiae). |
| AntibodyType | Monoclonal. |
| Clone | 38F3 |
| OtherNames | rRNA2"-O-methyltransferasefibrillarin;34kDanucleolarsclerodermaantigen;FBL;FIB1;FLRN;Nop1p |
| Accession | P15646FBRL_YEAST;P22087FBRL_HUMAN; |
| Producedin | Mouse |
| Purity | Concentratedcellculturesupernatant. |
| Applications | WesternBlotting(WB),Immunocytochemistry(IC).Adilutionof1:2000(celllysates)to1:10000(nuclearfractions)isrecommendedforWBofyeastproteinsamplesfollowedbychemiluminescentdetection(ECL).Forothernon-ECLWBmethods,adilutionof1:1000to1:5000isrecommended.Adilutionof1:500isrecommendedforECLWBonmammalianfibrillarin.Adilutionof1:1000to1:5000isrecommendedforIConyeastcellsand1:500formammaliancells.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB.Thisclonewasselectedbecauseitisspecificforthe~34kDaFibrillarin.Itstainsasinglebandonwesternblottingandshowsaclearandstrongpunctatestainingofyeastnuclei.Itcanthereforebeusedasamarkerfornucleoliinawidevarietyofspecies. |
| AntibodyAgainst | Fibrillarin(Nop1p) |
| Cross-reactivity | Human,Rat,Drosophila,S.pombe,C.elegansandandS.pombe.Predictedtoreactwithothermammaliantissues. |
| Form | Lyophilized,withoutpreservatives. |
| Reconstitution | Reconstitutein250uLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | PaeschkeK.etal(2005)Telomereend-bindingproteinscontroltheformationofG-quadruplexDNAstructuresinvivo.NatStructMolBiol.2005Oct;12(10):847-54. PostbergJ.etal(2005)Synthesisofpre-rRNAandmRNAisdirectedtoachromatin-poorcompartmentinthemacronucleusofthespirotrichousciliateStylonychialemnae.ChromosomeRes.2006;14(2):161-75. DuT.G.etal(2008)NucleartransitoftheRNA-bindingproteinShe2isrequiredfortranslationalcontroloflocalizedASH1mRNA.EMBORep.2008Aug;9(8):781-7. SrivastavaL.etal(2010)MammalianDEADboxproteinDdx51actsin3"endmaturationof28SrRNAbypromotingthereleaseofU8snoRNA.MolCellBiol.2010Jun;30(12):2947-56. SasanoY.etal(2008)DistributionofU3smallnucleolarRNAandfibrillarinduringearlyembryogenesisinCaenorhaBDitiselegans.Biochimie.2008Jun;90(6):898-907. MengL.etal(2007)Nucleolartraffickingofnucleosteminfamilyproteins:commonversusprotein-specificmechanisms.MolCellBiol.2007Dec;27(24):8670-82. |
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