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| Description | GFAPisa50kDaintra-cytoplasmicfilamentousproteinoftheCytoskeletoninastrocytes.Duringthedevelopmentofthecentralnervoussystem,itisacell-specificMarkerthatdistinguishesastrocytesfromotherglialcells.GFAPimmunoreactivityhasbeenshowninimmatureoligodendrocytes,epiglotticcartilage,pituicytes,papillarymeningiomas,myoepithelialcellsofthebreastandinnon-CNS:Schwanncells,salivaryglandneoplasms,entericgliacells,andmetastasizingrenalcarcinomas.Atleast3isoformsareproducedfromalternatesplicing.TheseisoformsdifferintheC-terminalregionwhichisencodedbyalternativeexons.Thisantibodydetectsallthreeisoforms. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | GFAPfrompigspinalcord |
| Clone | GA-8 |
| OtherNames | Astrocyte;Glialfibrillaryacidicprotein; |
| Accession | P14136GFAP_HUMAN;Q8WP16Q8WP16_PIG |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanGFAPhasapredictedlengthof432residuesandMWof50kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetectGFAPinformalinfixedandparaffinembeddedtissuesaswellasformalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase. |
| References | 1.ReevesS.A,etal.Proc.Natl.Acad.Sci.U.S.A.86:5178-5182(1989). 2.BrennerM,etal.BrainRes.Mol.BrainRes.7:277-286(1990). 2.IsaacsA,etal.Genomics51:152-154(1998). 3.OtaT,etal.Nat.Genet.36:40-45(2004). 4.NielsenA.L,etal.J.Biol.Chem.277:29983-29991(2002). 5.SinghR,etal.Genomics82:185-193(2003). 6.BrennerM,etal.Nat.Genet.27:117-120(2001). 7.BrockmannK,etal.Eur.Neurol.50:100-105(2003). 8.StumpfE,etal.Arch.Neurol.60:1307-1312(2003). 9.SawaishiY,etal.Neurology58:1541-1543(2002). 10.AokiY,etal.Neurosci.Lett.312:71-74(2001). |
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