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| Description | GAP-43isexpressedbydevelopingandregeneratingneurons,andtoalesserextent,reactiveglialcells.Itisalsoamajorcomponentofneuronalgrowthcones.GAP-43isusedtospecificallylabelinjuredneuronsandtoscoreneuronalregeneration.ThisantibodyreactswiththethreeknownisoformsandtobothkinaseCphosphorylatedanddephosphorylatedformsofGAP-43. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | GAP-43proteinfromneonatalratforebrainmembranes |
| Clone | GAP-8A12 |
| OtherNames | Neuromodulin;GAP43;PP46;p57;B-50;F1;AxonalmembraneproteinGAP-43;Growth-associatedprotein43;NeuralphosphoproteinB-50; |
| Accession | P07936NEUM_RAT;P17677NEUM_HUMAN |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanGAP-43hasapredictedlengthof238residuesandMWof25kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectGAP-43informalinfixedandparaffinembeddedtissuesaswellasformalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat;chicken;snake |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase. |
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