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| Description | CD97antigenisareceptorinvolvedinbothadhesionandsignalingprocessesearlyafterleukocyteactivation.Italsoplaysanessentialroleinleukocytemigration.Theunprocessedprecursoris853aminoacidslongandhasapredictedmolecularweightof91.9KDa.CD97antigenisaheterodimerconsistingofalargeextracellularalphasubunitandaseven-transmembranebetasubunitthatarenon-covalentlylinked.ItcontainsfiveEGF-likedomainsandoneGPSdomain.Thisproteininteractswithcomplementdecay-acceleratingfactor(DAF)throughthefirsttwoEGFdomains,andwithchondroitinsulfate(onlyisoform1)throughthefourthEGFdomain.CD97antigenisbroadlyexpressedanditispresentonmosthematopoieticcells.Itisalsoabundantlyexpressedbysmoothmusclecells.ExpressionofthisproteinisincreasedunderinflammatoryconditionsintheCNSofmultiplesclerosisandinsynovialtissueofpatientsofrheumatoidarthritis.Thisproteinisrapidlyup-regulatedduringlymphocyteactivation.C97antigenbelongstotheG-proteincoupledreceptor2fmailyandtheLN-TM7subfamily. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | PartialrecombinantproteinofhumanCD97antigen(aminoacids421to529)withaGSTtag. |
| Clone | 5D5 |
| OtherNames | LeukocyteantigenCD97 |
| Accession | CD97_HUMAN |
| Producedin | Mouse |
| Purity | ProteinGpurifiedimmunoglobulin |
| Applications | WB,directandsandwichELISAarerecommendedforthisantibody.InsandwichELISAthisantibodyhasadetectionlimitof0.1ng/mlforrecombinantGSTtaggedCD97antigen.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | SpecificityofthisantibodyhasbeenconfirmedbyWBandELISAagainstthepartialrecombinant,GSTtagged,humanCD97antigen. |
| Cross-reactivity | Human.Otherspecieshavenotyetbeentested. |
| Blastit | Ifyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteins,followthelinkintheAccessionfield,selectthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
| Form | LyophilisedfromPBSpH7.2 |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforhigherstABIlityorat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
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