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Biosensis/Mouse monoclonal antibody to human Vinculin [VIN-54]: IgG/M-1228-100/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Biosensis/Mouse monoclonal antibody to human Vinculin [VIN-54]: IgG/M-1228-100/100 µg

DescriptionVinculinisacytoskeletalproteinassociatedwithcell-cellandcell-matrixadherenstypejunctions.Multipleisoformsareproducedbyalternativesplicing.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenPurifiedVinculinfromhumanuterus
CloneVIN-54
OtherNamesMetavinculin;VCL;
AccessionP18206VINC_HUMAN;
ProducedinMouse
PurityIgG
ApplicationsImmunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.HumanVinculin(isoform2,Metavinculin)hasapredictedlengthof1,134residuesandaMWof124kDa.Aconcentrationof2.0-4.0µg/mlisrecommendedtodetecttheproteininformalin/acetonefixedfrozentissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen.
Cross-reactivityHuman;mouse;rat;chicken;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3
ReconstitutionReconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial.
StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
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