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| Description | Neural cell adhesion molecule (NCAM) is a glycoprotein with 5 extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. NCAM mediates cell-cell adhesion in neural cells as well as a variety of other cell types. NCAM is expressed widely during embryonic development but is restricted in the adult brain. Many isoforms are produced from the alternate splicing of a single gene with 26 exons. This antibody reacts with higher molecular weight human isoforms; NCAM 140 and NCAM 180. |
| Batch No. | See product label |
| Unit size | 100 µg |
| Antigen | Growth cone enriched plasma membrane fraction from E17 rat forebrain |
| Clone | IML-43 |
| Other Names | CD56; NCAM; N-CAM 140; NCAM-1; N-CAM-1; |
| Accession | P13591 NCAM1_HUMAN; P13596 NCAM1_RAT |
| Produced in | Mouse |
| Purity | IgG |
| Applications | Western Blotting (WB). A concentration of 4.0 µg/ml is recommended for WB. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity | The specificity of this antibody has been confirmed by WB against the antigen. |
| Cross-reactivity | Human; rat; |
| Blast it | If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form | Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3 |
| Reconstitution | Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material. |
| Storage | At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date | 12 months after purchase |
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