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| Description | Neurofilamentscontainthreeintermediatefilamentproteins:light(68kDa),medium(160kDa)andheavy(200kDa).Neurofilamentheavy(NF200orNF-H)isphosphorylatedanditisthoughtthatthisresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber. |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µl |
| Antigen | Fulllengthnativeprotein(purified)fromPigspinalcord. |
| AntibodyType | Monoclonal |
| Isotype | IgG1 |
| Clone | NAP4 |
| OtherNames | NF-200;NF200;NF-H;NEFH;N52;Neurofilamentheavypolypeptide;NeurofilamenttripletHprotein;200kDaneurofilamentprotein;KIAA0845;NFH; |
| Accession | P12036NFH_HUMAN; |
| Producedin | Mouse |
| Applications | WesternBlotting(WB),Immunocytochemistry(IC)andFlowCytometry(2ug/10^6cells).SuggesteddilutionforWBof1:5,000-10,000.ThisantibodyrecognisesNF-Hinfrozensections,tissuecultureandinformalin-fixedsections.SuggesteddilutionforICis1:500-1,000.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB.ThisantibodyrecognisesphosphorylatedNF-HKSP(lysine-serine-proline)typesequences.Insomespeciesthereissomecross-reactivitywiththerelatedKSPsequencesfoundinsubunitNF-M. |
| AntibodyAgainst | NeurofilamentHeavy,phosphorylated |
| Cross-reactivity | Chicken,Rat.Predictedtoreactwithmammalsduetosequencehomology. |
| BlastURL | Clickhere |
| Form | Lyophilisedwith5%trehalose |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | 1.BoylanK.etal(2009)ImmunoreactivityofthephosphorylatedaxonalneurofilamentHsubunit(pNF-H)inbloodofALSmodelrodentsandALSpatients:evaluationofbloodpNF-HasapotentialALSbioMarker.JNeuRochem.2009Dec;111(5):1182-91. 2.RangarajuS.etal(2009)Moleculararchitectureofmyelinatedperipheralnervesissupportedbycalorierestrictionwithaging.AgingCell.2009Apr;8(2):178-91. |
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