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| Description | Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
Replacedby | M-1391-50 | BatchNo. | Seeproductlabel | Unitsize | 100µg | Antigen | Pigspinalcord | Clone | NF-68 | OtherNames | NF-L;NF68;NEFL;Neurofilamentlightpolypeptide;NFL; | Accession | P02547NFL_PIG;P07196NFL_HUMAN | Producedin | Mouse | Purity | IgG | Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.PigNF68hasapredictedlengthof549aaandMWof68kDa.Aconcentrationof2.0-4.0µg/mlisrecommendedtodetectNF68informalinfixedandparaffinembeddedtissuesaswellasformalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. | Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. | Cross-reactivity | Human;pig; | Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. | Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 | Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. | Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. | ExpiryDate | 12monthsafterpurchase. | SpecificReferences | JohnsonVEetal.(2016)SNTFimmunostainingrevealspreviouslyundetectedaxonalpathologyintraumaticbraininjury.ActaNeuropatho.131:115-35.doi:10.1007/s00401-015-1506-0.Application:IHC,FFPEtissuewithheat-mediatedantigenretrieval.Species:Humanandswine. |
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