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| Description | The High Mobility Group (HMG)-box conserved domain is found in proteins that promote chromatin function and gene regulation, assist with transcription, replication, recombination and DNA repair. High mobility group protein B4 (HMGB4) contains two HMG-box DNA-binding domains. |
| Batch No. | See product label |
| Unit size | 100 µg |
| Antigen | A synthetic peptide RNKFKEQQPNTYVGFKEF which differs by a single residue to a region (24-41) from human High mobility group protein B4 (HMGB4). To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
| Other Names | HMGB4; |
| Accession | Q8WW32 HMGB4_HUMAN; |
| Produced in | Rabbit |
| Purity | Affinity purified on antigen column |
| Applications | Western Blotting (WB). A concentration of 1.0-2.0 µg/ml is recommended for WB. Human HMGB4 has a predicted length of 186 residues and MW of 22 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity | The specificity of this antibody has been confirmed by WB against the antigen. |
| Cross-reactivity | Human; mouse; rat; |
| Blast it | If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form | Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 |
| Reconstitution | Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Storage | At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. |
| Expiry Date | 12 months after purchase |
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