企业QQ
官方微信
2"-OMe-ibu-G-CE Phosphoramidite5"-Dimethoxytrityl-N-isobutyryl-Guanosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1320-10 | 2"-OMe-ibu-G-CE Phosphoramidite | 1.0 g | £160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3120-02 | 2"-OMe-ibu-G-CE Phosphoramidite | 0.25 g | £50.00£47.50Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3120-05 | 2"-OMe-ibu-G-CE Phosphoramidite | 0.5 g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3120-90 | 2"-OMe-ibu-G-CE Phosphoramidite | 100 umole | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
2"-OMe-ibu-G-CE Phosphoramidite5"-Dimethoxytrityl-N-isobutyryl-Guanosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
The use of UltraMild monomers in oligonucleotide synthesis has allowed very sensitive dyes like TAMRA, HEX and Cy5 to be used virtually routinely. The DNA and RNA monomers are currently available and we also provide this set of 2"-OMe-RNA monomers. In our version of this chemistry, we use as protecting groups phenoxyacetyl (Pac) for A, acetyl (Ac) for C, and isopropyl-phenoxyacetyl (iPr-Pac) for G.It has become clear that acetic anhydride in the conventional capping mix can cause transamidation in situations where an amine protecting group is quite labile. This leads to acetyl protection on the amino group that may be slow to be removed. Consequently, if many dG residues are included in the oligonucleotide, we recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix.
Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 3-5 days
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-ibu-G-CE Phosphoramidite5"-Dimethoxytrityl-N-isobutyryl-Guanosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
MSDS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-ibu-G-CE Phosphoramidite5"-Dimethoxytrityl-N-isobutyryl-Guanosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Glen Report 19.2: New Lines Of Monomers - Ht For High Throughput And Lc For Low Cost Rn
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-ibu-G-CE Phosphoramidite5"-Dimethoxytrityl-N-isobutyryl-Guanosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3120-02 | 0.25grams | .25grams | 2.87 | 82.33 | 49.4 | 30.88 | 22.45 | 16.47 | 4.12 |
| 10-3120-05 | 0.5grams | .5grams | 5.75 | 178.33 | 107 | 66.88 | 48.64 | 35.67 | 8.92 |
| 10-3120-10 | 1.0gram | 1grams | 11.49 | 369.67 | 221.8 | 138.63 | 100.82 | 73.93 | 18.48 |
| 10-3120-90 | 100µmoles | .087grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3120-2HT | 2grams | 2grams | 22.99 | 753 | 451.8 | 282.38 | 205.36 | 150.6 | 37.65 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3120-02 | 0.25grams | .25grams | 4.29 | .07 | 79.4 | 49.63 | 36.09 | 4.96 | |
| 10-3120-05 | 0.5grams | .5grams | 8.58 | .07 | 165.2 | 103.25 | 75.09 | 10.33 | |
| 10-3120-10 | 1.0gram | 1grams | 17.16 | .07 | 336.8 | 210.5 | 153.09 | 21.05 | |
| 10-3120-90 | 100µmoles | .087grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-3120-2HT | 2grams | 2grams | 34.31 | .07 | 679.8 | 424.88 | 309 | 42.49 | |
| Mermade | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3120-02 | 0.25grams | .25grams | 4.29 | .07 | 81 | 50.63 | 36.82 | ||
| 10-3120-05 | 0.5grams | .5grams | 8.58 | .07 | 166.8 | 104.25 | 75.82 | ||
| 10-3120-10 | 1.0gram | 1grams | 17.16 | .07 | 338.4 | 211.5 | 153.82 | ||
| 10-3120-90 | 100µmoles | .087grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-3120-2HT | 2grams | 2grams | 34.31 | .07 | 681.4 | 425.88 | 309.73 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn
