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Chemical Phosphorylation Reagent
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1900-02 | Chemical Phosphorylation Reagent | 0.25g | £128.00£121.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1900-90 | Chemical Phosphorylation Reagent | 100µmoles | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Chemical Phosphorylation Reagent
| Chemical Phosphorylation Reagent |
| 2-[2-(4,4"-Dimethoxytrityloxy)ethylsulfonyl]ethyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C34H45N2O7PS | M.W.: 656.77 | F.W.: 79.98 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved.(Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions.In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.Note: The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
Chemical Phosphorylation Reagent is most commonly used tophosphorylate the 5’-terminus of an oligonucleotide. Although this product isalso successful in 3’-phosphorylation, 3’-Phosphate CPG allows directpreparation of oligonucleotides with a 3’-phosphate group. ChemicalPhosphorylation Reagent II contains a DMT group on a side chain which is stableto base cleavage and can be left on the oligonucleotide for use in RPpurification. The DMT group is later removed with aqueous acid and the sidechain is eliminated after brief treatment with aqueous ammonium hydroxide toyield the 5’-phosphate.1 Solid CPR II is similar in performance to CPR II but itis easier to prepare aliquots since it is a powder. Many researchers treatsynthesis supports with a hindered base (e.g., diethylamine,diisopropylethylamine, or DBU) post-synthesis to eliminate and remove thecyanoethyl phosphate groups. In this way, the acrylonitrile formed in situ isremoved from the support and is not available to alkylate dT residues at the N3position in the oligos. Since the sulfonylethyl group in 3’-Phosphate CPG isalso susceptible to ß-elimination leading to oligo cleavage, this technique isnot compatible with 3’-phosphate CPG. Using CPR II CPG, which is base labile butdoes not support ß-elimination, the cyanoethyl groups can be removed from theoligo prior to cleavage and base deprotection. ABI-style vials and columns aresupplied unless otherwise requested.
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Chemical Phosphorylation Reagent
Material Safety Data Sheet
| Glen Report 8.2: 5"-PHOSPHORYLATION - NOW COMPATIBLE WITH DMT-ON PURIFICATION |
| Glen Report 12.1: PRODUCT UPDATE - WHICH CHEMICAL PHOSPHORYLATION REAGENT? |
| Glen Report 20.1: New Products |
| Glen Report 23.1: Technical Brief - Chemical Phosphorylation Ð considering the options |
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Chemical Phosphorylation Reagent
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 3.81 | 113.67 | 68.2 | 42.63 | 31 | 22.73 | 5.68 |
| 10-1900-90 | 100µmoles | .066grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 5.68 | .07 | 107.2 | 67 | 48.73 | 6.7 | |
| 10-1900-90 | 100µmoles | .066grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 5.68 | .07 | 108.8 | 68 | 49.45 | ||
| 10-1900-90 | 100µmoles | .066grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
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