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dA-Thiophosphoramidite
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1700-02 | dA-Thiophosphoramidite | 0.25g | £336.00£319.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1700-90 | dA-Thiophosphoramidite | 100µmoles | £140.00£133.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
dA-Thiophosphoramidite
| dA-Thiophosphoramidite |
| 5"-Dimethoxytrityl-N-benzoyl-2"-deoxyAdenosine,3"-[(Β-thiobenzoylethyl)-(1-pyrrolidinyl)]-thiophosphoramidite | ||
| Formula: C51H51N6O7PS2 | M.W.: 955.09 | F.W.: 345.34(dithioate) |
Diluent: 10% (v/v) Anhydrous Dichloromethane in Anhydrous Acetonitrile |
| Coupling: See Technical Bulletin |
| Deprotection: See Technical Bulletin for details (Technical Bulletin). |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: No data available |
Replacing two non-bridging oxygen atoms with sulfur atoms in a DNA phosphodiester linkage creates a phosphorodithioate (PS2) linkage.1 Like natural DNA, the phosphorodithioate linkage is achiral at phosphorus. This analog is completely resistant to nuclease degradation and forms complexes with DNA and RNA with somewhat reduced stabilities.2 Moreover, it has been found that PS2-ODNs bind proteins with a higher affinity than their phosphodiester analogues2-6 suggesting that PS2-ODNs may have additional utility in the form of sulfur-modified phosphate ester aptamers (thioaptamers)3,6-8 for therapeutic and diagnostic applications. Thiophosphoramidites are now commercially available after recent work at AM Biotechnologies (www.thioaptamer.com).
A typical cycle for the solid-phase synthesis of a PS2 linkage is different from a standard cycle for the synthesis of normal phosphate linkages. After coupling, the resulting thiophosphite triester is then sulfurized with DDTT. Capping is carried out AFTER sulfurization.
Upon completion of the automated synthesis, deprotection is carried out using a concentrated ammonia:ethanol (3:1, v:v) mix containing 20 mM DTT at 55 °C for 15-16 h.
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dA-Thiophosphoramidite
MSDS
Glen Report 20.1: THIOPHOSPHORAMIDITES AND THEIR USE IN SYNTHESIZING OLIGONUCLEOTIDE PHOSPHORODITHIOATE LINKAGES
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dA-Thiophosphoramidite
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.15M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1700-02 | 0.25grams | .25grams | 1.75 | 45 | 27 | 16.88 | 12.27 | 9 | 2.25 |
| 10-1700-90 | 100µmoles | .096grams | .67 | 9 | 5.4 | 3.38 | 2.45 | 1.8 | .45 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1700-02 | 0.25grams | .25grams | 2.6 | .1 | 45.6 | 28.5 | 20.73 | 2.85 | |
| 10-1700-90 | 100µmoles | .096grams | 1 | .1 | 13.6 | 8.5 | 6.18 | .85 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1700-02 | 0.25grams | .25grams | 2.6 | .1 | 47.2 | 29.5 | 21.45 | ||
| 10-1700-90 | 100µmoles | .096grams | 1 | .1 | 15.2 | 9.5 | 6.91 | ||
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