SampleTyple | Plasma,Serum,Cellculturesupernatant,otherbodyfluid |
Components | |
Method | Colorimetricmethodat570nm |
StandardCurve | |
Sensitivity | 20µM-1000µM |
ReactionTime | 30minutes |
Applications | ForBIOLOGicalresearch: |
Storage | -80°C |
Notes | Materialneededbutnotsupplied Aplatereadercapableofmeasuringabsorbanceat570nm AdjustablePipettesandarepeatpipettor Distilledwater(milliQorHPLC-grade) ClearFlatbottom96-wellplatesifnotincludedinthekitpurchased |
Shipping | Icepacks |
ReagentPreparation Note:Allreagentsarefrozen.Werecommendyouspinsmallvialsbeforeopening. 1.GalactoseAssayBuffer(10x) Note:Pleasedon’tdodilutionintheprovidedGalactoseAssayBufferbottleitself. Thebottlecontains10mlof10xGalactoseAssayBuffer.Theassaybuffermustbeequilibratedtoroomtemperature.Pleasemakeappropriateamountof1xGalctoseAssayBufferfortheassay,forexample,bydiluting1mlof10xGalactoseAssayBufferwith9mlofdistilledwater.Prepareadditionaldilutedassaybufferifneeded.Storeat-80?C. 2.GalactoseStandard(10x) Note:Pleasedon’tdodilutionintheprovidedGalactoseStandardvialitself. Thevialcontains100µlof10mMGalactoseStandard.Thestandardmustbeequilibratedtoroomtemperaturebeforeuse.Dilute100µlof10mMGalactoseStandardwith900µlofdilutedGalactoseAssayBuffertopreparea1mMGalactoseStandard.1mlofdilutedGalctoseStandardisenoughformaking3standardcurvesifassayedinduplicate.Storeat-80?C. 3.AssayProbe Thevialcontains45μlofAssayProbe.Theassayprobemustbeequilibratedtoroomtemperaturebeforeuse.Itissufficientfor100wells.Protectfromlight.Storeat-80?C. 4.GalactoseEnzymeMix Thevialcontains35μlofGalctoseEnzymeMix.Theenzymemixmustbethawedonicebeforeuse.Itissufficientfor100wells.Storeat-80?C. |
Protocol 1.SamplePreparation Serum,plasma,urine,otherbodyfluidscanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;…)toensurethereADIngsarewithinthestandardcurverange.YoursamplescanbedilutedwithdH2OordilutedGalactoseAssayBuffer. Note: |
2.StandardCurvePrepation Add50μl,40μl,30μl,20μl,10μl,5μl,1μl,and0μlofdilutedGalactoseStandardtoeachwell,thenadjustvolumeto50μl/wellwithdilutedGalactoseAssayBuffer. |
3.ReactionSolutionPreparation 6µlGalactoseAssayEnzymeMix 8µlAssayProbe 1mlofReactionSolutionisenoughfor~20wells. PrepareappropriateamountofReactionSolutionforthenumberofassaystobeperformed. Note:PleasedonotkeepanyleftoverReactionSolutionasithastobemadefreshrightbeforeyourunassays.IfoldReactionSolutionisused,assayswillnotwork. |
4.Performtheassay a.Mixthereactionsolutionwellandadd50μlofthereactionsolutiontoeachwellcontainingtheGalactosestandardsandtestsamples. |
5.Calculation a.AveragetheOD570nmvaluesofreplicatewellsofeachGalactoseStandard,testsamples,andblank.Then,correctbackgroundbysubtractingthevaluederivedfromtheblankfromallsamplereadings. Galactose(μM)=[(Correctedabsorbance)-(y-intercept)]/Slope |
MaterialSafetyDataSheet
Date:Oct26,2015
1.PRODUCTANDCOMPANYIDENTIFICATION
1.1Productidentifier
Productname:GalactoseAssayKit
CatalogNo:1200091002,120009100P
1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst
Forresearchuseonly.
1.3Detailsofthesupplierofthesafetydatasheet
Company:EtonBioscienceInc
TollFree:1-800-758-1630
Tel:1-800-758-1630
Fax:1-800-507-2912
1.4Emergencytelephonenumber
1-800-758-1630
2.HAZARDSIDENTIFICATION
2.1Classificationofthesubstanceormixture
Notahazardoussubstanceormixture
2.2GHSLabelelements,includingprecautionarystatements
Notahazardoussubstanceormixture
2.3Otherhazards
None
3.COMPOSITION/INFORMATIONONINGREDIENTS
3.1Substances
GalactoseAssayBuffer | GalactoseAssayEnzymeMix | |||||||
Components | CAS# | Components | CAS# | |||||
PotassiumPhosphateMonobasic | 7778-77-0 | PotassiumPhosphateMonobasic | 7778-77-0 | |||||
PotassiumPhosphateDibasic | 7758-11-4 | PotassiumPhosphateDibasic | 7758-11-4 | |||||
Water | 7732-18-5 | GalactoseOxidase | 9028-79-9 | |||||
GalactoseStandard | AssayProbe | |||||||
Components | CAS# | Components | CAS# | |||||
SodiumD-Galactose | 59-23-4 | DMSO | 67-68-5 | |||||
PotassiumPhosphateMonobasic | 7778-77-0 | |||||||
PotassiumPhosphateDibasic | 7758-11-4 | |||||||
Water | 7732-18-5 | |||||||
EyeProtection | Wearsafetygoggles |
SkinProtection | Wearprotectiveclothing |
HandProtection | Wearprotectivegloves |
Respiratoryprotection | WearNIOSH/MSHAapprovedrespirators |
9.PHYSICALANDCHEMICALPROPERTIES
9.1Informationonbasicphysicalandchemicalproperties
GalactoseAssayBuffer
Appearance | Liquid,colorless |
pH | 7.0 |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
GalactoseStandard
Appearance | Liquid,colorless |
pH | 7.0 |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
GalactoseAssayEnzymeMix
Appearance | Liquid,yellow |
pH | Nodataavailable |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
AssayProbe
Appearance | Liquid,colorless |
pH | Nodataavailable |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
9.2Othersafetyinformation
Nodataavailable.
10.STABILITYANDREACTIVITY
10.1Reactivity
Nodataavailable
10.2Chemicalstability
Stableunderrecommendedstorageconditions.
10.3Possibilityofhazardousreactions
Nodataavailable.
10.4Conditionstoavoid
Nodataavailable
10.5IncompatIBLematerials
Strongoxidizingagents
10.6Hazardousdecompositionproducts
Hazardousdecompositionproductsformedunderfireconditions:carbonoxides
11.TOXICOLOGICALINFORMATION
11.1Informationontoxicologicaleffects
Toxicologicaleffectsonthisproducthavenotbeenthoroughlystudied.
12.ECOLOGICALINFORMATION
12.1Toxicity
Avoidreleaseintoenvironment
12.2Persistenceanddegradability
Nodataavailable
12.3.Bioaccumulativepotential
Nodataavailable
12.4Mobilityinsoil
Nodataavailable
12.5ResultsofPBTandvPvBassessment
Nodataavailable
12.6Otheradverseeffects
Nodataavailable
13.DISPOSALINFORMATION
13.1Wastetreatmentmethods
Disposeinaccordancewithlocal,state,andfederalregulations.
14.TRANSPORTINFORMATION
DOT:
Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
IATA:
Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
ADR/RID
Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
15.REGULATORYINFORMATION
EURiskandSafetyphrases:
R36/37/38:irritatingtoeyes/respiratorysystem/skin
16.OTHERINFORMATION
Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
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