Everest Biotech/Glucose Assay Kit II-100 Assays w/Reader Plate(1x)/120007100P/100 Assays w/Reader Plate(1x)

SampleTyple	Plasma,Serum,Cellculturesupernatant,otherbodyfluid
Components
Method	Colorimetricmethodat570nm
StandardCurve
Sensitivity	4µM-200µM
ReactionTime	30minutes
Applications	ForBIOLOGicalresearch: Glucosemeasurementinbiologicalsamples Fordrug/pharmresearch: DruginfluenceonGlucosemetabolism
Storage	-80°C
Notes	Materialneededbutnotsupplied Aplatereadercapableofmeasuringabsorbanceat570nm AdjustablePipettesandarepeatpipettor Distilledwater(milliQorHPLC-grade) ClearFlatbottom96-wellplatesifnotincludedinthekitpurchased
Shipping	Icepacks

MaterialSafetyDataSheet

Date:Oct26,2015

1.PRODUCTANDCOMPANYIDENTIFICATION

1.1Productidentifier

Productname:GlucoseAssayKitII

CatalogNo:1200071002,120007100P

1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst

Forresearchuseonly.

1.3Detailsofthesupplierofthesafetydatasheet

Company:EtonBioscienceInc

TollFree:1-800-758-1630

Tel:1-800-758-1630

Fax:1-800-507-2912

1.4Emergencytelephonenumber

1-800-758-1630

2.HAZARDSIDENTIFICATION

2.1Classificationofthesubstanceormixture

Notahazardoussubstanceormixture

2.2GHSLabelelements,includingprecautionarystatements

Notahazardoussubstanceormixture

2.3Otherhazards

None

3.COMPOSITION/INFORMATIONONINGREDIENTS

3.1Substances

ReagentPreparation Note:Allreagentsarefrozen.Werecommendyouspinsmallvialsbeforeopening. 1.GlucoseAssayBuffer(10x) Note:Pleasedon’tdodilutionintheprovidedGlucoseAssayBufferbottleitself. Thebottlecontains10mlof10xGlucoseAssayBuffer.Theassaybuffermustbeequilibratedtoroomtemperature.Pleasemakeappropriateamountof1xGlucoseAssayBufferfortheassay,forexample,bydiluting1mlof10xGlucoseAssayBufferwith9mlofdistilledwater.Prepareadditionaldilutedassaybufferifneeded.Storeat-80?C. 2.GlucoseStandard(10x) Note:Pleasedon’tdodilutionintheprovidedGlucoseStandardvialitself. Thevialcontains100µlof2mMGlucoseStandard.Thestandardmustbeequilibratedtoroomtemperaturebeforeuse.Dilute100µlof2mMGlucoseStandardwith900µlofdilutedGlucoseAssayBuffertopreparea200μMGlucoseStandard.1mlofdilutedGlucoseStandardisenoughformaking3standardcurvesifassayedinduplicate.Storeat-80?C. 3.AssayProbe Thevialcontains45μlofAssayProbe.Theassayprobemustbeequilibratedtoroomtemperaturebeforeuse.Itissufficientfor100wells.Protectfromlight.Storeat-80?C. 4.GlucoseEnzymeMix Thevialcontains70μlofGlucoseEnzymeMix.Theenzymemixmustbethawedonicebeforeuse.Itissufficientfor100wells.Storeat-80?C.

Protocol 1.SamplePreparation Blood(serum/plasma),urine,otherbodyfluidscanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;…)toensurethereADIngsarewithinthestandardcurverange.YoursamplescanbedilutedwithdH2OordilutedGlucoseAssayBuffer. Add50μlofsamplestoeachwell. Werecommendthatsamplesbeassayedinduplicate. Note: Ifpreparedsamplesarenotassayedthesameday,storethesamplesat-80?C.Thesampleswillbestableforonemonthwhilestoredat-80?C.Forfrozensamples,dilutionsofsamplesmustbedonerightbeforeassaying.

2.StandardCurvePrepation Add50μl,40μl,30μl,20μl,10μl,5μl,1μl,and0μlofdilutedGlucoseStandardtoeachwell,thenadjustvolumeto50μl/wellwithdilutedGlucoseAssayBuffer. WerecommendthatGlucoseStandardsbeassayedinduplicate.Astandardcurvehastoberunineachassay.

3.ReactionSolutionPreparation 50μlReactionMixisrequiredforeachwelland1mlofReactionSolutionispreparedwiththefollowings: 980µlGlucoseAssayBuffer(1x) 12µlGlucoseAssayEnzymeMix 8µlAssayProbe 1mlofReactionSolutionisenoughfor~20wells. PrepareappropriateamountofReactionSolutionforthenumberofassaystobeperformed. Note:PleasedonotkeepanyleftoverReactionSolutionasithastobemadefreshrightbeforeyourunassays.IfoldReactionSolutionisused,assayswillnotwork.

4.Performtheassay a.Mixthereactionsolutionwellandadd50μlofthereactionsolutiontoeachwellcontainingtheGlucosestandardsandtestsamples. b.Incubatethereactionfor30minutesina37?Cincubator. c.Measureabsorbanceat570nminamicroplatereader.

5.Calculation a.AveragetheOD570nmvaluesofreplicatewellsofeachGlucoseStandard,testsamples,andblank.Then,correctbackgroundbysubtractingthevaluederivedfromtheblankfromallsamplereadings. b.MakeastandardcurvebyplottingOD570nmvaluesfromeachGlucoseStandardsasafunctionofGlucoseconcentration.Thiscanbedonewithexcelspreadsheet. c.CalculatethevalueofGlucoseinsamplesusingtheequationobtainedfromthelinearregressionofthestandardcurve. Glucose(μM)=[(Correctedabsorbance)-(y-intercept)]/Slope

4.FIRSTAIDMEASURES

4.1Descriptionoffirstaidmeasures

Eyecontact

Checkforandremovecontactlensesandflushwithcopiousamountsofwater;assureadequateflushingbyseparatingtheeyelidswithfingers;callaphysician

SkinContact

Flushwithcopiousamountsofwater;removecontaminatedclothingandshoes;callaphysician

Inhalation

Removetofreshair.Ifnotbreathinggiveartificialrespiration;callaphysician

Ingestion

Ifswallowed,nevergiveanythingbymouthtoanunconsciousperson.Rinsemouthwithwater;callaphysician

4.2Mostimportantsymptomsandeffects,bothacuteanddelayed

Noinformationavailable

4.3Indicationofanyimmediatemedicalattentionandspecialtreatmentneeded.

Noinformationavailable

5.FIREFIGHTINGMEASURES

5.1Extinguishingmedia

Suitableextinguishingmedia

Waterspray,alcohol-resistantfoam,drychemicalorcarbondioxide

5.2Specialhazardsarisingfromthesubstanceormixture

Noinformationavailable

5.3Adviceforfirefighters

Wearself-containedbreathingapparatusandprotectiveclothingtopreventcontactwithskinandeyes.

6.ACCIDENTALRELEASEMEASURES

6.1Personalprecautions,protectiveequipmentandemergencyprocedures

Usefullpersonalprotectiveequipment.Evacuatepersonneltosafeareas.Avoidbreathingvapors,mistorgas.

6.2Environmentalprecautions

Preventfurtherleakageorspillage.Preventproductfromenteringdrain.

6.3Methodsandmaterialforcontainmentandcleaningup

Keepinsuitable,closedcontainersfordisposal

7.HANDLINGANDSTORAGE

7.1Precautionsforsafehandling

Avoidskin/eyecontact.Useprotectiveequipmentasneeded.Washcontaminatedclothingbeforereuse.

7.2Conditionsforsafestorage,includinganyincompatibilities

Keepcontainertightlyclosed.Storeat-80?C.

7.3Specificenduse(s)

Nodataavailable.

8.EXPOSURECONTROLS/PERSONALINFORMATION

8.1ControlParameters

Thisproductcontainsnohazardousmaterialswithoccupationalexposurelimits.

8.2Exposurecontrols

Engineeringcontrols

Provideshowerandeyewashstation

Personalprotectiveequipment

	GlucoseAssayBuffer				GlucoseAssayEnzymeMix
	Components		CAS#		Components	CAS#
	PotassiumPhosphateMonobasic		7778-77-0		PotassiumPhosphateMonobasic	7778-77-0
	PotassiumPhosphateDibasic		7758-11-4		PotassiumPhosphateDibasic	7758-11-4
	Water		7732-18-5		GlucoseOxidase	9001-37-0
	GlucoseStandard				AssayProbe
	Components		CAS#		Components	CAS#
	SodiumD-Glucose		50-99-7		DMSO	67-68-5
	PotassiumPhosphateMonobasic		7778-77-0
	PotassiumPhosphateDibasic		7758-11-4
	Water		7732-18-5

EyeProtection	Wearsafetygoggles
SkinProtection	Wearprotectiveclothing
HandProtection	Wearprotectivegloves
Respiratoryprotection	WearNIOSH/MSHAapprovedrespirators

9.PHYSICALANDCHEMICALPROPERTIES

9.1Informationonbasicphysicalandchemicalproperties

GlucoseAssayBuffer

GlucoseStandard

GlucoseAssayEnzymeMix

AssayProbe

9.2Othersafetyinformation

Nodataavailable.

10.STABILITYANDREACTIVITY

10.1Reactivity

Nodataavailable

10.2Chemicalstability

Stableunderrecommendedstorageconditions.

10.3Possibilityofhazardousreactions

Nodataavailable.

10.4Conditionstoavoid

Nodataavailable

10.5IncompatIBLematerials

Strongoxidizingagents

10.6Hazardousdecompositionproducts

Hazardousdecompositionproductsformedunderfireconditions:carbonoxides

11.TOXICOLOGICALINFORMATION

11.1Informationontoxicologicaleffects

Toxicologicaleffectsonthisproducthavenotbeenthoroughlystudied.

12.ECOLOGICALINFORMATION

12.1Toxicity

Avoidreleaseintoenvironment

12.2Persistenceanddegradability

Nodataavailable

12.3.Bioaccumulativepotential

Nodataavailable

12.4Mobilityinsoil

Nodataavailable

12.5ResultsofPBTandvPvBassessment

Nodataavailable

12.6Otheradverseeffects

Nodataavailable

13.DISPOSALINFORMATION

13.1Wastetreatmentmethods

Disposeinaccordancewithlocal,state,andfederalregulations.

14.TRANSPORTINFORMATION

DOT:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
IATA:

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

ADR/RID

Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport

15.REGULATORYINFORMATION

EURiskandSafetyphrases:

R36/37/38:irritatingtoeyes/respiratorysystem/skin

16.OTHERINFORMATION