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LeptinisahormonesecretedfromwhiteADIpocytesandplaysimportantroleintheregulationoffoodintakeandenergybalance.LeptinfunctionsviasignalingpathwaysinvolvingOB-Rinhypothalamus.Inmammals,leptinismainlyproducedbytheadiposetissueandencodesbodyfatreserves,actingasashort-termsatietysignal.Infish,thepresenceofaleptin-likepeptidewasfirstevidencedbyimmuno-cross-reactivity[14],anditsexistencewascertainlydemonstratedafterthefindingbysyntenyofaleptinsequenceinthepufferfish.
| Source | E.coli |
| Description | RecombinantCarassiusauratusLeptinisproducedbyourYeastexpressionsystemandthetargetgeneencodingPro22-Cys171isexpressedwitha8HistagattheN-terminus. |
| Names | Leptin,ObeseProtein,ObesityFactor,LEP,OB,OBS |
| Accession# | B8YI02 |
| Formulation | Lyophilizedfroma0.2μmfilteredsolutionofPBS,pH7.4. |
| Shipping | Theproductisshippedatambienttemperature. |
| Reconstitution | Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting. Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100μg/ml. DissolvethelyophilizedproteininddH2O. Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles. |
| Storage | Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks. Reconstitutedproteinsolutioncanbestoredat4-7°Cfor2-7days. Aliquotsofreconstitutedsamplesarestableat<-20°Cfor3months. |
| Purity | Greaterthan95%asdeterminedbyreducingSDS-PAGE. |
| Endotoxin | Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest. |
| AminoAcidSequence | HHHHHHHHPVHPDRLKNMVKLQADTIILRIKDHNEKLKLYPKLLIGDPELYPEVPADRHIQGLGSIMDTLTIFQKVLQRLPKGHVSQIRSDLSTLLGYLKERTTSMHCILKEPANGRSLDAFLEENATHHITLGYLALDRLKQFMQKLIVNLDQLKSC |
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