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Everest Biotech/Recombinant Dechloromonas aromatica (Strain RCB) Chlorite Dismutase (N-6His) 1mg/3712960012/1mg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Everest Biotech/Recombinant Dechloromonas aromatica (Strain RCB) Chlorite Dismutase (N-6His) 1mg/3712960012/1mg

Chloritedismutase(Cld)foundinprokaryoticorganisms,alsoknownasChloriteO2-lyase,isab-typehemecontainingenzymethatcatalyzesthereductionofchloriteintochlorideplusdioxygen.ThesubunitofchloritedismutaseconsistsofahemefreeN-terminalandahemebcontainingC-terminalferredoxin-likefoldwithhighstructuralhomologytothedye-decolorizingperoxidases(DyPs).ThephysiologicalroleofCldinprokaryotehasbeenshownthatsomemicroorganismscanuseperchlorateorchlorateasterminalelectronacceptorsforanaerobicrespirationtherebyproducingchloritethatmustbedetoxified.Thisenzymehasgainedattentionbecauseitcanbeusedinthedevelopmentofbioremediationprocesses,biosensors,andcontrolleddioxygenproduction.

Source

E.coli

Description

RecombinantDechloromonasaromatica(strainRCB)ChloritedismutaseChloritedismutaseisproducedbyourE.coliexpressionsystemandthetargetgeneencodingMet35-Asp282isexpressedwitha6HistagattheN-terminus.

Names

Chloritedismutase,ChloriteO(2)-lyase,Daro_2580,Cld

Accession#

Q47CX0

Formulation

Lyophilizedfroma0.2μmfilteredsolutionofPBS,0.5mMEDTA,pH7.4.

Shipping

Theproductisshippedatambienttemperature.

Reconstitution

Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting.
Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100μg/ml.
DissolvethelyophilizedproteininddH2O.
Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles.

Storage

Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks.
Reconstitutedproteinsolutioncanbestoredat4-7°Cfor2-7days.
Aliquotsofreconstitutedsamplesarestableat<-20°Cfor3months.

Purity

Greaterthan95%asdeterminedbyreducingSDS-PAGE.

Endotoxin

Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest.

AminoAcidSequence
MGSSHHHHHHSSGLVPRGSHMENLYFQGMQPMQSMKIERGTILTQPGVFGVFTMFKLRPDWNKVPVAERKGAAEEVKKLIEKHKDNVLVDLYLTRGLETNSDFFFRINAYDLAKAQTFMREFRSTTVGKNADVFETLVGVTKPLNYISKDKSPGLNAGLSSATYSGPAPRYVIVIPVKKNAEWWNMSPEERLKEMEVHTTPTLAYLVNVKRKLYHSTGLDDTDFITYFETDDLTAFNNLMLSLAQVKENKFHVRWGSPTTLGTIHSPEDVIKALAD