Chloritedismutase(Cld)foundinprokaryoticorganisms,alsoknownasChloriteO2-lyase,isab-typehemecontainingenzymethatcatalyzesthereductionofchloriteintochlorideplusdioxygen.ThesubunitofchloritedismutaseconsistsofahemefreeN-terminalandahemebcontainingC-terminalferredoxin-likefoldwithhighstructuralhomologytothedye-decolorizingperoxidases(DyPs).ThephysiologicalroleofCldinprokaryotehasbeenshownthatsomemicroorganismscanuseperchlorateorchlorateasterminalelectronacceptorsforanaerobicrespirationtherebyproducingchloritethatmustbedetoxified.Thisenzymehasgainedattentionbecauseitcanbeusedinthedevelopmentofbioremediationprocesses,biosensors,andcontrolleddioxygenproduction.
Source | E.coli |
Description | RecombinantDechloromonasaromatica(strainRCB)ChloritedismutaseChloritedismutaseisproducedbyourE.coliexpressionsystemandthetargetgeneencodingMet35-Asp282isexpressedwitha6HistagattheN-terminus. |
Names | Chloritedismutase,ChloriteO(2)-lyase,Daro_2580,Cld |
Accession# | Q47CX0 |
Formulation | Lyophilizedfroma0.2μmfilteredsolutionofPBS,0.5mMEDTA,pH7.4. |
Shipping | Theproductisshippedatambienttemperature. |
Reconstitution | Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting. |
Storage | Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks. |
Purity | Greaterthan95%asdeterminedbyreducingSDS-PAGE. |
Endotoxin | Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest. |
AminoAcidSequence | MGSSHHHHHHSSGLVPRGSHMENLYFQGMQPMQSMKIERGTILTQPGVFGVFTMFKLRPDWNKVPVAERKGAAEEVKKLIEKHKDNVLVDLYLTRGLETNSDFFFRINAYDLAKAQTFMREFRSTTVGKNADVFETLVGVTKPLNYISKDKSPGLNAGLSSATYSGPAPRYVIVIPVKKNAEWWNMSPEERLKEMEVHTTPTLAYLVNVKRKLYHSTGLDDTDFITYFETDDLTAFNNLMLSLAQVKENKFHVRWGSPTTLGTIHSPEDVIKALAD |