Deprecated: Creation of dynamic property cls_session::$session_data_table is deprecated in /www/sites/www.188bio.com/index/systems/cls_session.php on line 49
Everest Biotech/Recombinant E. coli RNA Pyrophosphohydrolase/rppH 50μg/3712990501/50μg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

Everest Biotech/Recombinant E. coli RNA Pyrophosphohydrolase/rppH 50μg/3712990501/50μg

MessengerRNA(mRNA)degradationplaysakeyroleinthecontrolofgeneexpressioninallorganismsbylimitingthenumberoftimesthateachmRNAmoleculecanbeusedasatemplateforproteinsynthesis.RNApyrophosphohydrolase,alsocalledRppH,isamasterregulatorof5"-dependentmRNAdecay.Itacceleratesthedegradationoftranscriptsbyremovingpyrophosphatefromthe5"-endoftriphosphorylatedRNA,leADIngtoamorelABIlemonophosphorylatedstatethatcanstimulatesubsequentribonucleasecleavage.RppHpreferentiallyhydrolyzesdiadenosinepenta-phosphatewithATPasoneofthereactionproducts,andcanbeabletohydrolyzediadenosinehexa-andtetra-phosphate.However,thisproteinhasnoactivityondiadenosinetri-phosphate,ADP-ribose,NADHandUDP-glucose.InthemeningitiscausingstrainE.coliK1,ithasbeenshowntoplayaroleinHBMEC(humanbrainmicrovascularendothelialcells)invasioninvitro.

Source

E.coli

Description

RecombinantE.coliRNApyrophosphohydrolaseisproducedbyourE.coliexpressionsystemandthetargetgeneencodingMet1-Gly176isexpressed.

Names

RNApyrophosphohydrolase,(Di)nucleosidepolyphosphatehydrolase,Ap5Apyrophosphatase,rppH

Accession#

P0A776

Formulation

Suppliedasa0.2μmfilteredsolutionof50mMTris,500mMNaCl,10%glycerol,pH8.0.

Shipping

Theproductisshippedondryice/icepacks.

Storage

Storeat<-20°C,stablefor6monthsafterreceipt.
Pleaseminimizefreeze-thawcycles.

Purity

Greaterthan95%asdeterminedbyreducingSDS-PAGE.

Endotoxin

Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest.

AminoAcidSequence
MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGESAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLLQLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQENTPKPQNASAYRRKRG