MessengerRNA(mRNA)degradationplaysakeyroleinthecontrolofgeneexpressioninallorganismsbylimitingthenumberoftimesthateachmRNAmoleculecanbeusedasatemplateforproteinsynthesis.RNApyrophosphohydrolase,alsocalledRppH,isamasterregulatorof5"-dependentmRNAdecay.Itacceleratesthedegradationoftranscriptsbyremovingpyrophosphatefromthe5"-endoftriphosphorylatedRNA,leADIngtoamorelABIlemonophosphorylatedstatethatcanstimulatesubsequentribonucleasecleavage.RppHpreferentiallyhydrolyzesdiadenosinepenta-phosphatewithATPasoneofthereactionproducts,andcanbeabletohydrolyzediadenosinehexa-andtetra-phosphate.However,thisproteinhasnoactivityondiadenosinetri-phosphate,ADP-ribose,NADHandUDP-glucose.InthemeningitiscausingstrainE.coliK1,ithasbeenshowntoplayaroleinHBMEC(humanbrainmicrovascularendothelialcells)invasioninvitro.
Source | E.coli |
Description | RecombinantE.coliRNApyrophosphohydrolaseisproducedbyourE.coliexpressionsystemandthetargetgeneencodingMet1-Gly176isexpressed. |
Names | RNApyrophosphohydrolase,(Di)nucleosidepolyphosphatehydrolase,Ap5Apyrophosphatase,rppH |
Accession# | P0A776 |
Formulation | Suppliedasa0.2μmfilteredsolutionof50mMTris,500mMNaCl,10%glycerol,pH8.0. |
Shipping | Theproductisshippedondryice/icepacks. |
Storage | Storeat<-20°C,stablefor6monthsafterreceipt. |
Purity | Greaterthan95%asdeterminedbyreducingSDS-PAGE. |
Endotoxin | Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest. |
AminoAcidSequence | MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGESAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLLQLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQENTPKPQNASAYRRKRG |