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Everest Biotech/Recombinant E. coli Tryptophan Synthase β Chain/Trp B 1mg/3713020012/1mg

Tryptophansynthaseisanenzymethatcatalyzesthefinaltwostepsinthebiosynthesisoftryptophan.ItiscommonlyfoundinEubacteria,Archaebacteria,Protista,Fungi,andPlantae,butisabsentfromanimalssuchashumans.Tryptophansynthasetypicallyexistsasanα-ββ-αcomplex.ThealphasubunitisresponsIBLeforthealdolcleavageofindoleglycerolphosphatetoindoleandglyceraldehyde3-phosphate:L-serine+1-C-(indol-3-yl)glycerol3-phosphate=L-tryptophan+D-glyceraldehyde3-phosphate+H2O.Thebetasubunitscatalyzetheirreversiblecondensationofindoleandserinetoformtryptophaninapyridoxalphosphate(PLP)dependentreaction.Theirassemblyintoacomplexleadstostructuralchangesinbothsubunitsresultinginreciprocalactivation.

Source

E.coli

Description

RecombinantE.coliTryptophansynthasebetachainisproducedbyourE.coliexpressionsystemandthetargetgeneencodingThr2-Ile397isexpressedwitha6HistagattheN-terminus.

Names

Tryptophansynthasebetachain,trpB

Accession#

P0A879

Formulation

Lyophilizedfroma0.2μmfilteredsolutionofPBS,pH7.4.

Shipping

Theproductisshippedatambienttemperature.

Reconstitution

Alwayscentrifugetubesbeforeopening.Donotmixbyvortexorpipetting.
Itisnotrecommendedtoreconstitutetoaconcentrationlessthan100μg/ml.
DissolvethelyophilizedproteininddH2O.
Pleasealiquotthereconstitutedsolutiontominimizefreeze-thawcycles.

Storage

Lyophilizedproteinshouldbestoredat<-20°C,thoughstableatroomtemperaturefor3weeks.
Reconstitutedproteinsolutioncanbestoredat4-7°Cfor2-7days.
Aliquotsofreconstitutedsamplesarestableat<-20°Cfor3months.

Purity

Greaterthan95%asdeterminedbyreducingSDS-PAGE.

Endotoxin

Lessthan0.1ng/µg(1IEU/µg)asdeterminedbyLALtest.

AminoAcidSequence

MHHHHHHTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQFNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILKARGEI