FpgisaDNAglycosylasethatreleasesdamagedbasespreferentiallyfromduplexDNA.IthasanassociatedclassIAP(apurinic/apyrimidinic)lyaseactivity.FpgCleavestheDNAbackbonetogenerateasingle-strandbreakatthesiteoftheremovedbase,TheC-O-Pbond3"totheapurinicorapyrimidinicsiteinDNAisbrokenbyabeta-eliminationreaction,leaving3"and5"phosphorylgroups.
Source | E.coli |
Names | Formamidopyrimidine-DNAGlycosylase,Fapy-DNAGlycosylase,DNA-(ApurinicorApyrimidinicSite)LyaseMutM,APLyaseMutM,mutM,fpg |
Accession# | A7ZTI6 |
Formulation | Suppliedasa0.2μMfilteredsolutionof20mMTris-HCl,100mMNaCl,1mMEDTA,1mMDTT,50%Glycerol,pH7.8 |
Shipping | Theproductisshippedondryice/icepacks. |
Storage | Storeat<-20°C,stablefor6monthsafterreceipt. Pleaseminimizefreeze-thawcycles. |
Purity | Greaterthan95%asdeterminedbySEC-HPLCandreducingSDS-PAGE. |
Endotoxin | Lessthan0.1ng/μg(1IEU/μg). |
AminoAcidSequence | MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQKLEHHHHHH |
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