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Nanocs/DBCO PEG Silane/PG2-DBSL-5k-2/50 mg

Description:

DBCO(dibenzocycolctyne)PEGSilanederivativescangoClickchemistryreactionwithoutaneedofanymetalcatalysts.Thestrain-promoted1,3-dipolarcycloadditionofcyclooctynesandazides,alsotermedastheCu-freeclickreaction,isabioorthogonalreactionthatenablestheconjugationoftwomoleculesinaqueoussolution.DBCOPEGderivativespossessfastkineticsandstABIlityinaqueousbuffer.DBCOreagentscanbeusedtolabelazide-modifiedbiomoleculesspontaneouswithouttheneedfortoxicCucatalysts.PEGylationcanincreasesolubilityandstabilityandreduceimmunogenicityofpeptidesandproteins.Itcanalsosuppressthenon-specificbindingofchargedmoleculestothemodifiedsurfaces.

PhysicalProperties:

  • Whitesolidorviscousliquiddependsonmoleculeweight;
  • Solubleinregularaqeoussolutionaswellasmostorganicsolvents;

Copper-freeClickreactionprocedures:

  • Preparetheazide-containingreagentsinreactionbuffer.
  • AddDBCOreagentstoazidecontainingreagents.Add1molequivalentoflimitingreagentto1.5~3.0molequivalentsofabundancereagent.
  • Incubatethereactionatroomtemperaturefor2~4hoursor2~12hoursat40C.
  • Purifyconjugateseitherbydialysisorsize-exclusionchromatograph.

StorageConditions:

  • Storeat-200C,dessicate.Protectfromlight.Avoidfrequentthawandfreeze.
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