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Nanocs/DBCO PEG Folic acid/PG2-DBFA-5k/5 mg

Description:

DBCOPEGFolicacid,alsocalledDBCOPEGfolate,isoneofNanocs"folicacidPEGderivativesthatcangoClickchemistryreactionwithoutaneedofanymetalcatalysts.Thestrain-promoted1,3-dipolarcycloadditionofcyclooctynesandazides,alsotermedastheCu-freeclickreaction,isabioorthogonalreactionthatenablestheconjugationoftwomoleculesinaqueoussolution.DBCOPEGfolatederivativespossessfastkineticsandstABIlityinaqueousbuffer.DBCOreagentscanbeusedtolabelazide-modifiedbiomoleculesspontaneouswithouttheneedfortoxicCucatalysts.Folicacid,isalsoknownasvitaminM,vitaminB9orpteroyl-L-glutamicacid.FolicacidisanessentialbioactivemoleculefornumerousBIOLOGicalfunctions.Itparticipatesinthesynthesis,repairingandmethylationofDNAaswellastoactasacofactorinmanybiologicalreactions.DBCOfunctionalfolicacidcanbeeasilyattachedtoazidefunctionalizedmolecules,particlesorsolidsurfaces.

PhysicalProperties:

  • Yellowsolid;
  • SolubleinDMSO;

Copper-freeClickreactionprocedures:

  • Preparetheazide-containingreagentsinreactionsolution.
  • AddDBCOreagentstoazidecontainingreagents.Add1molequivalentoflimitingreagentto1.5~3.0molequivalentsofabundancereagent.
  • Incubatethereactionatroomtemperaturefor2~4hoursor2~12hoursat40C.
  • Purifyconjugateseitherbydialysisorsize-exclusionchromatograph.

StorageConditions:

  • Storeat-200C.Desicate.Protectfromlight.Avoidfrequentthawandfreeze.
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