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Physical Properties:
Storage Conditions:
Reaction Procedures:
Generally, a 5- to 10-fold molar excess ofPEG aldehyde over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
Reaction Steps:
Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. AddPEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.
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