Nanocs/DBCOPEG2000Rhodamine B/PG2DBRB2k/5 mg

Description:

DBCO(Dibenzocyclooctyne)isacycloalkynethatcanreactswithazidesviastrain-promoted1,3-dipolarcycloadditioninaqueoussolution,abioorthogonalreactionalsotermedCu-freeclickreaction. Thisreactionhasexcellentselectivityandbiocompatibility,suchthatthecomplimentaryreagentscanformcovalentbondswithrichlyfunctionalizedBIOLOGicalsystems.TheCu-freeclickreactionhasbeenapowerfultoolincatalyst-freebioconjugation.DBCOreagentspossesfastkinetcsandstABIlityinaqueousbufferandtheycanbeusedtolabelazide-modifiedbiomoleculeswithhighspecificityandreactivity. 

PhysicalProperties:

• Yellow/pale-yellowsolidorsemi-solid;
• SolubleinDMSOorDMF;

StorageConditions:

• Storeat-20⁰C,desiccate.Avoidfrequentthawandfrozen.

Copper-freeClickReactionProcedures:

Preparetheazide-containingsampleinreactionbuffer.AddDBCOconjugatetoazidecontainingsample.Recommendation:Add1moleequivalentoflimitingreagentto1.5-3.0moleequivalentsofhighestabundancereagent.Incubatethereactionatroomtemperaturefor2-4hoursor2-12hoursat4degreecelcius.Thereactionisnowreadyforpurification.