Nanocs/Silane PEG Amine, Silane PEG NH2/PG2AMSL3k/100 mg

Description:

Ethoxyormethoxylsilanefunctionalizedpolyethyleneglycol,silanePEG(PEG-Si)isasurfacereactivePEGderivativethatcanbeusedtomodifyglass,silicaandothersurfacesviathereactionbetweenhydroxylgroupandethoxyl/methoxylsilane.Pegylationcangreatlysuppressthenon-specificbindingofchargedmoleculestothemodifiedsurfaces.Andtheyhavewideapplicationsformedicadevice,biomemsorbiocompatIBLematerialdevelopment.

PhysicalProperties:

• Off-white/whitesolidorviscousliquiddependsonmoleculeweight;
• Solubleinregularaqeoussolutionaswellasmostorganicsolvents;

StorageConditions:

• Storeat-20⁰C,desiccated.SilanePEGtendstohydrolyzefrommoisture.Avoidfrequentthawandfrozen.

ReactionProcedures:

Generally,silanePEGisfirstdissolvedinamixtureofethanol/watersolution,thenallowthesolutiontocontactwithoxidizedglassorsilicasurfacefor30minto2hours.Underthiscondition,silanePEGshouldbindtothematerialcovalently.

MaterialsRequired:

• Pegylationsolution:Ethanol/water(w/w,95%/5%);
• SilanePEGstocksolution,10~50mg/mLinpegylationsolution,prepareinfresh.
• Washingsolution:Distilledwater.

ReactionSteps:

1.DissolvetargetedmaterialsinPegylationbuffer.

2.AddsilanePEGstocksolutiontothetargetedconjugationmaterialswiththefinalconcentrationkeepatleast10mg/mL.10~50molarexcessofsilanePEGneededforoptimalconjugation;

3.Allowmixtureagitatesatroomtemperaturefor30minatroomtemperatureor2hoursat4-20⁰C.

4.Washoutunreactedmaterialseitherbydistilledwater.