Nanocs/Succinimidyl acid PEG, mPEGNHS/PG1SPA1k/100 mg

Description:

N-hydroxylsuccinimide(NHS)functionalizedpolyethyleneglycol(PEG-NHS)isanamino(-NH₂)reactivePEGderivativethatcanbeusedtomodifyprotein,peptideoranyothersurfaceswiththeiravailableaminogroups.NHSestersreactwithprimaryaminegroupsatpH7~8.5toformstableamidebonds.ComparedtootherPEGNHSesterderivatives,oursuccinimidylcarbonate(SC)functionalizedmPEG-NHSofferssuperiorreactivityandhigherstabilityinaqueoussolution.NHSestersreactwithdeprotonatedprimaryamines,therefore,thereactionrequiresneutraltobasicpHvaluestoproceed.PrimaryaminesreactwithNHSestersbynucleophilicattackandNHSisreleasedasabyproduct.HydrolysisoftheNHS-estercompeteswiththereactioninaqueoussolutionandincreaseswithincreasingpH.

PhysicalProperties:

• Off-white/whitesolidorviscousliquiddependsonmoleculeweight;
• Solubleinregularaqeoussolutionaswellasmostorganicsolvents;

StorageConditions:

• Storeat-20⁰C,desiccate.NHSPEGtendstohydrolyzefrommoisture.Avoidfrequentthawandfrozen.

ReactionProcedures:

Generally,a10to50-foldmolarexcessofNHSPEGovertheamountofamine-containingmaterialresultsinsufficientconjugation.

MaterialsRequired:

• Conjugationbuffer:Sodiumbicarbonate100mMbuffer,pH8.5orotheramine-freebufferatpH7-8.5.
• PEGNHSstocksolution:100mgin1mLconjugationbuffer.
• Washingsolution:Distilledwateroranyaqueousbuffer.

ReactionSteps:

Dissolvetargetedmaterialsinconjugationbuffer.Estimatetheconcentrationofprimaryaminegroupsonthetargetedmaterials.AddNHSPEGstocksolutiontothetargetedconjugationmaterialswiththefinalconcentrationkeepatleast10mg/mL.10~50molarexcessofPEGNHSneededforoptimalconjugation;Allowmixtureagitatesatroomtemperaturefor30~60minatroomtemperatureor2hoursat4⁰C.Conjugatescanbepurifiedeitherbysizeexclusionchromatographyordialysis.