Nanocs/Aldehyde PEG Aldehyde,CHOPEGCHO/PG2AL10k/1 g

PEGAldehydeDescription:

AldeydePEGisanaminereactivePEGderivativethatcanbeusedtomodifybiomoleculesviaavailableaminegroups.Thereactionbetweenaldehydegroupwithε-amineoflysineresiduesandtheα-amineattheN-terminusproducesanintermediateSchiffbase.FurtherreductionwithhydridewillformastableC-Nbond.PEGaldehydesreactwithaminegroupsatapHoffrom5.5to9.5.HigherpHwillresultinmultiplePegylationwithbothterminalandlysinegroups.AldehydePEGisacommonlyusedPEGreagentforn-terminuspegylationinproteinsorpeptides.

PhysicalProperties:

• Off-white/whitesolidorviscousliquiddependsonmoleculeweight;
• Solubleinregularaqeoussolutionaswellasmostorganicsolvents;

StorageConditions:

• Storeat-20⁰C

ReactionProcedures:

Generally,a10-to50-foldmolarexcessofPEGaldehydeovertheamountofamine-containingmaterialresultsinsufficientconjugation.

MaterialsRequired:

• Pegylationbuffer:Amine-freeaqueousbuffer,pH6~8.5.
• PEGaldehydestocksolution:100mgin1mLconjugationbuffer.
• Washingsolution:Distilledwateroranyaqueousbuffer.

ReactionSteps:

Dissolvetargetedmaterialsinpegylationbuffer.Estimatetheconcentrationofprimaryaminegroupsonthetargetedmaterials.AddPEGaldehydestocksolutiontothetargetedconjugationmaterialswiththefinalconcentrationkeepatleast10mg/mL.Allowmixtureagitatesatroomtemperaturefor2~4hrsatroomtemperatureorovernight4⁰C.Afterreaction,C=Nbondcanbereducedbysodiumborohydride.Conjugatecanbepurifiedeitherbysizeexclusionchromatographyordialysis.